固相放射免疫法和胶体金法测定乙肝五项标志物的比较

Comparison of Solid Phase and Colloidal Gold Method in Determination of Five Serological Markers of Hepatitis B

目的比较固相放射免疫法(SPRIA)和胶体金法检测乙肝五项血清学指标,了解胶体金法测定HBV-M的准确性和可行性,观察SPRIA法测定HBV-M的临床应用价值。方法分别应用SPRIA法定量和胶体金法定性测定312例随机血清中的乙肝五项指标。结果 SPRIA法和胶体金法检测HBsAb和HBeAb结果差异有统计学意义(P<0.05、P<0.01),其余三项间差异均无统计学意义(P>0.05)。结论胶体金法快速、简单、敏感性和特异性高,适合HBV急诊筛查。SPRIA法对HBV感染的诊断和疗效观察以及指导乙肝疫苗的接种更有临床的意义。

Objective To compare solid phase radiation immune method and colloidal gold method in quantitative detection of five serological markers of hepatitis B,to understand the accuracy and feasibility of colloidal gold method in determining HBV-M,and to observe the clinical value of solid phase radiation immune method in determining HBV-M. Methods We applied solid phase radioimmunoassay quantificationally and colloidal gold method qualitatively separately to determine five serological markers of hepatitis B in 312 cases of random sera. Results There were statistically significant differences in the results of HBsAb and HBeAb between the two methods（P0.05,P0.01）,but no statistically significant difference was found in the other results（P0.05）. Conclusions Colloidal gold method is fast and simple,with high sensitivity and high specificity,which is suitable for screening for HBV in emergency department.Solid phase radioimmunoassay has more clinical significance in the diagnosis of HBV infection,observation of therapeutic effects and guidance of hepatitis B vaccine inoculation.