摘要
目的:探讨补肾填髓方促进去势兔骨髓间充质干细胞(MSC)s的成骨分化能力。方法:应用Van Kossa染色法和ELISA法对比补肾填髓方和成骨诱导剂对MSCs钙化结节和骨钙素的影响。结果:补肾填髓方能显著提高去势兔MSCs钙化结节数量,与去势组比较,差异有非常显著性意义(P<0.01),但作用较成骨诱导剂弱(P<0.05),同时也能显著提高骨钙素含量,与去势组比较,差异有显著性意义(P<0.05),钙化结节数量和骨钙素含量的增多与补肾填髓方用药时间呈正相关(P<0.01)。结论:补肾填髓方用于治疗骨质疏松症,与促进MSCs向成骨细胞分化相关,但其促进MSCs成骨的机制仍需进一步研究。
Objective: To investigate the effect of Bushen Tiansui Capsule on osteogenic differentiation of rabbit bone marrow mesenchymal stem cells (MSCs). Methods. Van Kossa staining method and enzyme linked immunosorbent assay (ELISA) were applied to investigate the osteogenic differentiation effect of Bushen Tiansui Capsule and osteogenic derivatives on the formation of calcified nodules and osteocalcin(OCN) content in ovariectomized rabbits. Results: Bushen Tiansui Capsule obviously elevated the number of calcified nodules and the content of OCN in MSCs of ovariectomized rabbits (P 〈 0.05 or P 〈 0.01), but the effect was weaker than that of'osteogenic derivatives(P 〈 0.05). The number of calcified nodules and the content of OCN were positively correlated with the medication time of Bushen Tiansui Capsule (P 〈 0.01). Conclusion, Bushen Tiansui Capsule can treat osteoporosis in clinically by improving osteogenic differentiation of MSCs. Its mechanism in improving the osteogenesis of MSCs still needs further research.
出处
《新中医》
CAS
北大核心
2011年第12期105-107,共3页
New Chinese Medicine
关键词
补肾填髓方
骨髓间充质干细胞
钙化结节
骨钙素
动物实验
兔
Bushen Tiansui Capsule
Bone Marrow Mesenchymal Stem Cells
Calcified Nodules
Osteocalcin
Animal Experiment
Rabbits