2009年至2011年新甲型H1N1流感病毒HA基因同源性比较与进化分析

Analysis of Homology and Evolution of HA Gene of 2009-2011 Novel Influenza (H1N1) Viruses

[目的]分析杭州市2009年1月至2011年1月新甲型H1N1流感病毒血凝素（HA）的基因序列,探讨该病毒的遗传变异和分子特性。[方法]应用RT-PCR的方法从临床标本中扩增新甲型H1N1流感病毒HA基因片段并测序。利用DNAMAN和Sequencher4.7软件对HA基因序列进行分析,比较HA基因氨基酸亲缘系数并绘制进化树。[结果]成功地对15例新甲型H1N1病毒的HA基因进行扩增并测序,2009年1月至2011年1月新甲型H1N1流感病毒的HA基因的氨基酸序列与参考株具有高度的同源性,其亲缘系数为98.9%~100%,且HA基因氨基酸序列发生了一定的变异。[结论]2009年至2011年新甲型H1N1流感病毒的基因组HA序列与我国和其他国家的新甲型H1N1流感病毒具有高度的同源性,但HA基因的氨基酸序列发生了变异,可能与患者的病情有关,因此需密切关注新甲型H1N1流感病毒的流行情况。

[Objective] To analyse the genome sequence of Hemagglutinin（HA） of the novel influenza A（H1N1） virus in Hangzhou from 2009 to 2011,and to investigate viral genetic characteristics and evolution status.[Methods] HA gene was amplified by using RT-PCR,the amplified products with expected size were submitted to direct sequencing.All the sequencing results were spliced with the software of DNAMAN and Sequencher 4.7.Alignments of HA gene together with corresponding sequences from all over the world were conducted to analyse the genetic distance and plot the phylogenetic tree of amino acids.[Results] Two segments of the HA gene were amplified and submitted for direct sequencing,15 samples were completed.After alignment,high homologies of 98.9% ~ 100% with amino acids of HA genes were observed among sequences of Influenza A（H1N1） virus all over the world.Although the HA gene homology is very high,however,some mutations were found among three years.[Conclusion] Genomes of 15 strains of novel influenza A（H1N1） viruses were sequenced and had a very high homology with the viruses originated from China and other countries.The mutations might be related to the symptoms of the patients,so that we should pay close attention to the epidemic information of the novel influenza A（H1N1） virus.