大蒜精油对糖尿病大鼠心肌细胞凋亡及PKCδ表达的影响

Garlic Refined Oil Influence on Rats'Cardiac Muscle Cell and PKCδ Expression

[目的]观察大蒜精油对糖尿病大鼠心肌细胞凋亡及PKCδ的影响。[方法]应用链脲佐菌素建立糖尿病大鼠模型,随机分为糖尿病模型组、大蒜精油低、中和高剂量组,同时设正常对照组。大蒜精油各剂量组按不同浓度比例喂饲大蒜精油。连续干预16d后测血糖水平,处死、剪取左心室组织,TUNEL检测心肌细胞凋亡情况,Western blotting检测PKCδ表达情况。[结果]模型组血糖升高明显、心肌细胞凋亡明显、PKCδ表达增加,大蒜精油各组情况得到改善。[结论]大蒜精油可有效降低糖尿病大鼠心肌PKCδ表达,抑制心肌细胞凋亡。

[Objective] To observe the garlic refined oil influence on diabetic rats＇ cardiac muscle cell apoptosis and PKCδ.[Method] Take streptozotocin to set up diabetic rats model,randomly divide them into model group,groups of low-,middle-and high-dosage garlic refined oil,meanwhile set a normal control group.After successive 16d,measure glucose level,capitalize and take ventriculus sinister tissue;take TUNEL to test cardiac muscle cell apoptosis;take western blotting to test PKCδ expression.[Result] In model group,the glucose was much raised,there＇s marked cardiac muscle cell apoptosis and increased PKCδ expression;the oil groups were all improved.[Conclusion] Garlic essential oil can effectively reduce diabetic rats cardiac muscle cell PKCδ expression and inhibit apoptosis.