正交试验法优选前列宁胶囊的壳聚糖纯化工艺

Optimization of the Chitosan Purification Process of Qianliening Capsuleby Orthogonal Test

[目的]筛选前列宁胶囊的最佳壳聚糖纯化工艺。[方法]采用天然絮凝剂壳聚糖纯化前列宁胶囊,运用正交设计,以浸膏得率、多糖保留率、大黄素为指标,考察壳聚糖浓度、反应温度、pH值和静置时间等影响因素,综合评价,筛选其最佳纯化工艺。[结果]最佳纯化工艺参数为:壳聚糖浓度20%,反应温度60℃,反应液pH=6,静置时间24h,提取液的出膏率大大降低。[结论]采用壳聚糖澄清剂纯化前列宁胶囊除杂效果好,制剂质量稳定。

[Objective]To screen the best purification technology of Qianliening capsule.[Method]The optimization purification technology of Qianliening capsule was investigated,which uses orthogonal design with the dry extract rate,the polysaccharide contain rate and emodin as index,to justify the influence of chitosan concentration,reaction temperature,PH and standing time.[Result]The optimal condition for the chitosan purification technology of Qianliening capsule was chitosan concentration 20%,reaction temperature 60 degrees,the reaction liquid pH7.0 and standing time 24h.According to the technological parameters of purification,the yield of extract is reduced greatly.[Conclusion]The chitosan clarification can be used for purifying Qianliening capsule.The quality of preparation is stable.