口腔黏膜下纤维化中细胞凋亡及Bcl-2和Bax的表达

Study on apoptosis and the expression of Bcl-2 or Bax in oral submucous fibrosis

目的:检测口腔黏膜下纤维化(OSF)组织中细胞凋亡状况及Bcl-2和Bax的表达,探讨OSF的发病机制。方法:采用TUNEL测定法、免疫组织化学SP法分别检测45例OSF和10例正常口腔黏膜组织中凋亡细胞的数量及Bcl-2、Bax的表达情况。结果:OSF晚期上皮中的凋亡指数明显高于正常对照组和OSF早、中期组(P<0.05),以空泡变性的棘层细胞为主;Bcl-2在OSF晚期组表达高于正常对照组(P<0.05),Bax在OSF晚期表达高于早期组(P<0.05);Bcl-2/Bax的比值与AI无直线相关关系(P>0.05)。结论:细胞凋亡可能是OSF晚期上皮萎缩的主要原因之一,Bcl-2、Bax参与了OSF的细胞凋亡,Bax在OSF晚期发挥重要作用。

Objective：To investigate the apoptosis and the expression of Bcl-2 or Bax in oral submucous fibrosis,so as to discuss the pathological mechanism of OSF.Method：TUNEL technique and immunohistochemical staining with SP method were used to detect the apoptosis and the expressions of Bcl-2 or Bax in 45 cases of OSF and 10 cases of normal oral mucosa speciments.Result：The AI in advanced stage of OSF was significantly higher than early,medium-term stages of OSF and normal oral mucosa（P 0.05）;The expression of Bcl-2 in advanced stage of OSF was higher than normal oral mucosa（P 0.05）,the expression of Bax increased at advanced stage of OSF compared with early stage of OSF（P 0.05）;There was no significant correlation between bcl-2/bax ratio and AI in all stages of OSF（P 0.05）.Conclusion：Excessive apoptosis may contribute to epithelial atrophy in advanced stage of OSF.The expression of Bcl-2 and Bax was was contributed to the apoptosis in OSF,overexpression of Bax may act as an important role in advanced stage of OSF.