摘要
基于Logistic和Leudeking-Piret方程,根据分批发酵过程中菌体生长、产物积累、总糖消耗、还原糖消耗及反应体系pH值的变化规律构建枯草芽孢杆菌E79发酵过程中菌体细胞生长、产物合成及基质消耗的动力学模型,应用SPSS11.5软件对数据进行计算与分析,Oirgin 7.5软件经非线性拟合与优化,获得了最佳模型参数值。结果表明:所构建的数学模型计算值与实验数据拟合良好,拟合模型R2均大于0.99,较好地反映了E79发酵过程中菌体细胞生长、基质消耗和木聚糖酶合成的动力学特征。枯草芽孢杆菌E79分批发酵过程中产物的合成属于生长偶联型。
The dynamic models of Bacillus subtilis E79 growth,xylanase formation and substrate consumption were constructed by using the Logistic and Leudeking-Piret equations based on the cell growth,product accumulation,total sugar consumption,reducing sugar consumption,and pH variation in the fermentation.The data were calculated and analyzed by SPSS 11.5 software.The optimal kinetics model parameters were obtained through optimization and non-linear curve fit by Origin 7.5 software.The results show that the models fit well with experimental data.The R2 of Bacillus subtilis E79 cells growth,xylanase formation and substrate consumption models are all greater than 0.99.These kinetic models can really reflect the characteristics of xylanase fermentation by Bacillus subtilis E79.Xylanase formation is completely associated with the growth of Bacillus subtilis E79 in all batch cultures.
出处
《化学工程》
CAS
CSCD
北大核心
2011年第11期6-9,20,共5页
Chemical Engineering(China)
基金
河南省高校科技创新杰出人才项目(2010HASTIT020)
