环孢素A对大鼠脊髓损伤早期Bcl-2与Bax表达的影响

Effect of Cyclosporine A on the Expression of Bcl-2 and Bax in the Early Period of Rat Spinal Cord Injury

目的探讨环孢素A(CsA)应用于脊髓损伤动物模型中对Bcl-2与Bax表达的影响。方法建立大鼠急性脊髓损伤实验动物模型。将108只SD大鼠随机分成单纯椎板切除组(对照组)、脊髓损伤组(损伤组),脊髓损伤后CsA治疗组(治疗组)。治疗组通过腹腔注射CsA;取受损节段脊髓行HE染色及免疫组织化学检测Bcl-2、Bax表达变化。结果损伤后2h损伤组和治疗组即有Bcl-2表达;损伤后24h Bcl-2表达达高峰;治疗组Bcl-2各时间点表达较损伤组高,差异有显著性(P<0.05)。损伤后2h损伤组和治疗组即有Bax表达;损伤后8hBax表达达高峰;损伤后72h Bax表达则降至基线水平。治疗组Bax各时间点表达较损伤组低,差异有显著性(P<0.05)。结论环孢素A治疗脊髓损伤能抑制Bax表达,促进Bcl-2表达,减轻脊髓继发性损伤。

Objective To investigate the Effect of of cyclosporine A on the expression of Bcl-2 and Bax in the early period of rat spinal cord injury.Methods An experimental rat model of spinal cord injury was established.108 rats were randomly divided into the control group,the injury group and the CsA-treated after injury group,Rats were sacrif iced and intubated for reperfusion at different time points after modeling.The structure of the rat spinal cord was shown by HE staining.The expression of Bcl-2and Bax in the rat spinal cord were detected by immunohistochemistry staining and analyzed quantitatively.Results The expression of Bcl-2 could be detected 2h after injury in the injury group and the CsA-treated group,reached its peak at 24h.After injury,the expressions of Bcl-2 protein at each study time point in CsA-treated group were signif icantly higher than injury group（P0.05）.The expression of Bax could be detected 2h after injury in the injury group and CsA-treated group,reached its peak at 8h.After injury,the expression of Bax protein at each study time point in CsA-treated group were signif icantly lower than injury group（P0.05）.Conclusions CsA depresses signif icantly the expression of Bax and promote signifi cantly the expression of Bcl-2 in the rat spinal cord after injury,relieves the secondary spinal cord injury.