纳米细菌纤维素柱层析分离

Column chromatographie separation of nano bacterial cellulose

以海南特有的椰子水为原料,通过木醋杆菌合成细菌纤维素(bacterial cellulose,简称BC)。通过膜分离将大分子凝胶和菌种以及破碎细胞除去,采用等电点法和Sevage法除蛋白、乙醚除脂肪、离子交换树脂除盐、DEAE-32柱进行柱层析得到中性纳米细菌纤维素,经过Sephadex-G100柱层析精制得到重均分子量(Mw)为9598,分子量分布指数约为1.1的纳米细菌纤维素。用透射电镜测试了其形貌,证明其颗粒近似球形,直径约20nm。

In present study,nano bacterial cellulose（NBC） was produced with Hainan coconut juice as the raw material by Acetobacter xylinum HN001.Membrane separations technology was employed to wipe off macromolecule hydrogels,strains and smudge cells.Then neutral NBC were obtained using Isoionic point and Sevage removing albumen,aether removing fat,ion exchange resin eliminating celite and DEAE-32 cellulose anion exchange chromatography.Finally,the NBC with weight-average molar mass（Mw） 9598 and polydispersity index about 1.1 were obtained by Sephadex-G100 glucose gel column chromatography.