Duchenne型肌营养不良患者肌肉组织myostatin mRNA的表达

Myostatin mRNA expression in patients of Duchenne muscular dystrophy

目的探讨肌肉生长抑制素myostatin基因mRNA在Duchenne型肌营养不良（Duchenne muscular dystrophy，DMD）肌肉组织中的表达变化。方法以甘油醛-3-磷酸脱氢酶基因mRNA表达水平作为内参照，采用逆转录-聚合酶链反应方法扩增7例DMD患者和4例非肌肉病对照者肌肉组织的myostatin mRNA，计算myostatin mRNA表达指数，进行半定量分析。结果两组肌肉组织均有myostatin mRNA表达；DMD患者肌肉组织中myostatin mRNA的表达指数为0．56±0．16，对照组为0．34±0．15，两组间差异有统计学意义（Z=-2．268，P=0．023）。结论DMD肌肉组织myostatin基因mRNA表达高于对照组水平，myostatin表达水平增高可能与DMD发病机制有关。

Objective To explore the expression of myostatin mRNA, a negative regulator of muscle growth, in the skeletal muscle of Duchenne muscular dystrophy （DMD） patients. Methods A semi-quantitative reverse transcription polymerase chain reaction was performed to evaluate the expression of myostatin in the skeletal muscle of 7 DMD patients and 4 healthy controls. Results The level of myostatin gene expression in the skeletal muscle of DMD patients was higher than that of healthy controls （0. 56 ± 0. 16 vs 0. 34 ± 0. 15, Z = - 2. 268,P = 0. 023）. Conclusions The myostatin gene expression was increased in the DMD patients compared to the healthy controls. Enhanced expression of myostatin in the skeletal muscle might be involved in the pathogenesis of DMD.