四川地区供浆员中肠道病毒71型中和抗体效价的分布

Distribution of Neutralizing Antibody Titer against Enterovirus 71 in Plasma Donors in Sichuan Region

目的通过检测EV71中和抗体效价(EV71-NT),对四川地区供浆员中EV71-NT的分布进行分析。方法应用基于细胞病变抑制的EV71-NT检测方法,检测四川地区349份健康人血浆的EV71-NT,同时用肠道病毒(EV)71型抗体诊断试剂盒(酶联免疫法)检测其EV71抗体,并分析二者的相关性;检测四川地区21 817份健康人血浆中EV71-NT的分布。结果该EV71-NT检测方法具有较好的重复性,测定高效价和低效价EV71中和抗体标准品的变异系数分别为30.9%和33.6%;该方法与ELISA法的相关性较差(r=0.024);四川地区21 817份健康人血浆中含高效价EV71中和抗体的比例在7.1%～18.4%之间,EV71高效价血浆占10.3%。结论在四川地区供浆员血浆中含有一定比例的高效价EV71中和抗体,可以直接从自然感染人群中筛选EV71抗体血浆进行EV71特异性免疫球蛋白的研制。

Objective To analyze the distribution of enterovirus 71 neutralizing antibody titer（EV7l-NT） in plasma donors in Sichuan Region. Methods A total of 349 healthy human plasma samples collected in Sichuan Region were determined for EV71-NT by a method developed based on the inhibition of CPE, and for EVT1 antibody by ELISA kit. The correlation between EV71-NT and EV7I antibody was analyzed, based on which the distribution of EV71-NT in 21 817 healthy human plasma samples collected in Siehuan Region was determined. Results The detection method for EV71-NT showed high reproducibility, by which the CVs of detection results of high and low titer standard neutralizing antibody were 30. 9% and 33.6% respectively. The method was lowly related to ELISA （r = 0. 024）. The percentage of high EV71-NT in 21 817 plasma samples collected in Sichuan Region was 7. 1% ～ 18. 4%, while that of high titer EVT1 antibody was 10. 3%. Conclusion A certain proportion of plasma collected from donors in Sichuan Region contains high EV71-NT, therefore human EV71 immnnoglobulin may be prepared with the EV71 antibody-positive plasma collected from naturally infected population.