丝瓜提取物抗氧化活性的研究

Antioxidative activity of extracts from Luffa cylindrica

目的:分离提取丝瓜抗氧化活性成分,观察其抗氧化效应。方法:应用溶剂萃取法及葡聚糖凝胶柱层析法分离提取丝瓜抗氧化活性物质,通过测定不同提取物的抗氧化能力,筛选出丝瓜的抗氧化活性成分。结果:由溶剂萃取法获得丝瓜提取物Ⅱ、Ⅲ、Ⅳ、Ⅴ,其中提取物Ⅲ抑制DPPH.和OH.自由基效果显著。提取物Ⅲ经柱层析进一步分离,得到A、B、C、D、E 5种组分,其中组分D、E抑制DPPH.和OH.自由基效果最显著,经鉴定提取物符合黄酮类物质特性。结论:丝瓜具有清除自由基的作用,且主要作用物质为黄酮类物质。

AIM： To isolate the active compounds of Luffa cylindrica and investigate their antioxidative activity.METHODS： Antioxidants from Luffa cylindrica were isolated using solvent extraction and Sephadex column chromatography.Active compounds of the extracts were identified with the antioxidative activity assays.RESULTS： With solvent extracting procedure,four extracts（Ⅱ,Ⅲ,Ⅳ and Ⅴ） were obtained.Among these extracts,extract Ⅲ had significant antioxidative activity,which exerted a potent scavenging effect for both DPPH· and OH· assays.For further purification,extract Ⅲ was subject to Sephadex column and five fractions（A,B,C,D and E） were isolated.Fraction D and E showed good efficiency on scavenging DPPH· and OH·,and exhibited flavanoid characteristics.CONCLUSION： Luffa cylindrica efficiently scavenges free radical species,and flavanoid exerts this action.