CCK-8对LPS诱导树突状细胞成熟的影响

Effect of CCK-8 on maturation of dendritic cells stimulated by LPS

目的:观察八肽胆囊收缩素(CCK-8)对脂多糖(LPS)诱导树突状细胞(DCs)成熟的影响。方法:采用粒-巨噬细胞集落刺激因子(GM-CSF)诱导法培养小鼠骨髓来源DCs,在LPS诱导其成熟过程中用不同浓度的CCK-8进行干预,采用流式细胞分析技术检测DCs表面主要组织相容性复合物II(MHC II)、分化群80(CD80)和分化群86(CD86)的表达;ELISA法检测DCs培养上清中白细胞介素1β(IL-1β)、白细胞介素6(IL-6)和肿瘤坏死因子α(TNF-α)的含量;MTT法检测CCK-8处理DCs对同种异体T细胞增殖反应的影响。结果:CCK-8剂量依赖性地抑制LPS诱导DCs表面CD80、CD86和MHC II表达(P<0.01,P<0.05);CCK-8抑制LPS诱导DCs分泌IL-1β、IL-6和TNF-α(P<0.01);并且,CCK-8降低LPS诱导DCs刺激同种异体T淋巴细胞增殖的活性(P<0.05,P<0.01)。结论:CCK-8对LPS诱导DCs成熟过程中的细胞表型、细胞因子分泌和抗原提呈功能有抑制作用,提示CCK-8有可能在抗感染和抵抗自身免疫性疾病过程中发挥重要调节作用。

AIM： To investigate the effect of cholecystokinin octapeptide（CCK-8） on the maturation of dendritic cells（DCs） stimulated by lipopolysaccharide（LPS）.METHODS： Mouse bone marrow-derived DCs were induced by granulocyte-macrophage colony-stimulating factor（GM-CSF）.When stimulated with LPS,DCs were exposed to different doses of CCK-8.The expression levels of major histocompatibility complex Ⅱ（MHC Ⅱ）,cluster of differentiation 80（CD80） and cluster of differentiation 86（CD86） on DCs were determined by flow cytometry.The levels of interleukin-1β（IL-1β）,interleukin-6（IL-6） and tumour necrosis factor-α（TNF-α） in the supernatants were detected by ELISA.The effect of CCK-8-treated DCs on the proliferation of allogenic T cells was analyzed by MTT method.RESULTS： CCK-8 inhibited the expression of MHC Ⅱ,CD80 and CD86 in LPS-stimulated DCs in a dose-dependent manner.CCK-8 decreased the production of IL-1β,IL-6 and TNF-α from DCs stimulated by LPS.Moreover,CCK-8 impaired the effect of LPS-stimulated DCs on the proliferation of allogenic T cells.CONCLUSION： CCK-8 effectively inhibits the phenotypic and functional maturation of DCs stimulated by LPS,suggesting that CCK-8 may play an important role in anti-infection and anti-autoimmune diseases.