摘要
目的:探讨siRNA沉默NADPH氧化酶1(Nox1)基因表达对胃癌细胞的生长抑制作用。方法:合成Nox1 siRNA,采用实时定量PCR和Western blotting观察Nox1 siRNA转染后胃癌SGC-7901细胞Nox1 mRNA及相应蛋白表达的变化;用CCK-8细胞增殖实验、流式细胞检测技术分别检测胃癌SGC-7901细胞增殖及凋亡的变化。结果:转染Nox1 siRNA后的胃癌SGC-7901细胞Nox1 mRNA及蛋白表达明显受抑制。与对照组相比,干扰组SGC-7901细胞生长明显变缓(P<0.05),细胞凋亡率明显增高(P<0.01)。结论:Nox1 siRNA可明显下调靶基因Nox1的表达,在体外可抑制胃癌SGC-7901细胞的生长并促进其凋亡。
AIM： To investigate the inhibitory effect of synthetic NADPH oxidase 1（Nox1） short interfering RNA（siRNA） on the growth of human gastric cancer SGC-7901 cell line.METHODS： The siRNA targeting Nox1 mRNA was transfected into SGC-7901 cells and Nox1 expression was determined by real-time PCR and Western blotting.Cell proliferation and apoptosis were examined by the methods of CCK-8 and flow cytometry,respectively.RESULTS： Nox1 siRNA significantly inhibited the expression of Nox1 in gastric cancer cells at both mRNA and protein levels.The apoptotic rate of SGC-7901 cells transfected with siRNA was significantly higher than that of the control cells（P0.01）.The proliferation of SGC-7901 cells was also significantly suppressed in vitro（P0.05） by siRNA transfection.CONCLUSION： Direct inhibition of Nox1 expression by siRNA significantly suppresses the proliferation of SGC-7901 cells in vitro,indicating a new approach of gene therapy for gastric cancer.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2011年第11期2229-2232,共4页
Chinese Journal of Pathophysiology
基金
江西省科技厅科技支撑计划资助项目(No.2010BSB00205)
江西省卫生厅资助项目(No.20091153)
