HLA-G和IL-10在非小细胞肺癌组织中的表达

Expression of the Human Leukocyte Antigen G and Interleukin-10 in Human Non-Small Cell Lung Cancer

目的:探讨HLA-G和白细胞介素(IL)-10在非小细胞肺癌组织中的表达状况及与各因素的关系。方法:以经手术切除的38例非小细胞肺癌组织标本为病例组,12例正常肺组织为对照组,通过免疫组化的方法检测2组标本的HLA-G和IL-10蛋白的表达水平,并对两者进行相关分析。结果:38例非小细胞肺癌组织标本中有28例(74%)HLA-G表达阳性,有30例(79%)IL-10表达阳性。HLA-G的表达水平与肺癌的TNM分期呈正相关(rs=0.672,P<0.01)。IL-10的表达水平与各临床病理因素无关。正常肺组织中无HLA-G和IL-10的表达。28例HLA-G阳性的肺癌组织中有25例(93%)IL-10阳性,两者呈正相关(rs=0.424,P<0.01)。结论:HLA-G和IL-10在肿瘤中相互作用形成了一个自分泌的恶性循环,而且破坏宿主的免疫功能最终使得肿瘤逃脱宿主免疫监视。

Objective：To investigate the expression of the human leukocyte antigen G （HLA-G） and interleukin-10 （IL-10） in non-small cell lung cancer （NSCLC）,and the relationship between HLA-G and IL-10.Methods：The expressions of HLA-G and IL-10 were detected using immunohistochemical method in surgically resected specimens from 38 patients with NSCLC （patient group） and normal lung tissues from 12 patients without lung cancer （control group）.Results：There were 28 positive expressions of HLA-G （74%） and 30 IL-10 protein expressions （79%） in specimens of NSCLCs,respectively.A positive correlation was found between HLA-G expression and stages of TNM （rs=0.672,P 0.01）.No significant correlation was found between IL-10 expression and clinicopathological factors.There was no HLA-G and IL-10 protein expression in normal lung tissues.Furthermore,IL-10 protein expression was demonstrated in 25 （93%） of 28 HLA-G positive cases.There was a positive correlation between them （rs=0.424,P 0.01）.Conclusion：HLA-G and IL-10 form a vicious autocrine circle of immune response abrogation in cancer,by which tumors evade immune surveillance.