ADAM17-siRNA抑制人MCF-7乳腺癌细胞体外侵袭能力的研究

Inhibitory Effects of ADAM17-siRNA on the Invasion of Human MCF-7 Breast Cancer

目的:观察人MCF-7乳腺癌细胞中解聚素-金属蛋白酶17(ADAM17)的表达受抑制后对肿瘤细胞体外侵袭能力的影响。方法:构建可以表达针对ADAM17的小干扰RNA(siRNA)的干涉载体,转染人乳腺癌细胞系MCF-7。利用real-time PCR法检测肿瘤细胞内ADAM17 mRNA的表达水平,Western blot法检测ADAM17蛋白的表达,Transwell方法检测肿瘤细胞的体外侵袭能力。结果:ADAM17 mRNA及蛋白在MCF-7细胞中呈高表达,转染特异性ADAM17-siRNA后,其表达显著被抑制,同时细胞的体外侵袭能力也明显受到抑制。结论:ADAM17及特异性的siRNA有望成为抗肿瘤侵袭治疗的新方法。

Objective：To investigate the effect of a disintegrin and metalloproteinase 17 （ADAM17）-siRNA on the invasion of human MCF-7 breast cancer cells.Methods：ADAM17-siRNA was transfected into MCF-7 breast cancer cells mediated by oligofectamine.ADAM17 mRNA and protein expressions were detected by real-time PCR and Western blot respectively,and the invasive ability of MCF-7 cells was studied by Transwell method.Results：ADAM17 mRNA and protein showed higher expression in MCF-7 cells,while it was significantly decreased with ADAM17 siRNA treatment.Furthermore,the invasive ability of MCF-7 cells was markedly inhibited by ADAM17 siRNA.Conclusion：ADAM17 plays an important role in the invasion of MCF-7 breast cancer cells,and ADAM17-siRNA can inhibit the invasive ability of MCF-7 cells by blocking the expression of ADAM17.