聚合高分子电解质分离纯化蛋白质

Polyelectrolyte as vehicles for isolation and purification of protein: a review

聚合高分子电解质含有大量阳离子或阴离子,通过静电作用结合带相反电荷的蛋白质,生成聚合高分子电解质-蛋白质复合物,电解质-蛋白质复合物通过桥连作用或疏水作用形成沉淀颗粒;聚合高分子电解质的选择性沉淀作用受电解质的分子量、添加剂量、溶液离子强度和pH的影响。用高分子电解质从大规模的低浓度溶液中选择性地沉淀目的蛋白质,为生物工程的下游处理开辟了一条新途径。

Polyelectrolyte with a large number of cations or anions could precipitate the oppositely charged proteins to form polyelectrolyte-protein complexes,which then aggregated to form larger particles via electrostatic attraction or hydrophobic interaction.The precipitation was affected by the molecular weight and concentration of the polyelectrolyte as well as the ionic strength and pH of the solution.The use of precipitation is an efficient method for selective separation of proteins from crude biological mixtures in the downstream processes of bioengineering.