一个水稻矮秆突变体的遗传分析及基因定位

Genetic Analysis and Mapping of Rice Dwarf Mutant ds1

水稻(Oryza sativa)是我国重要的粮食作物之一。水稻矮秆材料的引入掀起了第1次"绿色革命"。但近年来,在水稻育种中矮生基因遗传单一的问题越来越突出,已经严重影响到水稻产量的持续提高。利用60Co-γ射线辐照籼稻亲本材料M804获得了一个性状能够稳定遗传的矮秆突变体MU101。对该矮秆突变体和台粳16号杂交获得的F2代的遗传分析表明,该矮秆性状受1对隐性单基因控制,并暂命名为ds1。利用已有的SSR分子标记将DS1基因定位在水稻第5号染色体上,通过扩大群体和开发新的Indel标记,进一步将DS1基因定位在2个Indel标记之间,两者间的物理距离大约为384kb。该研究为DS1基因的克隆及其在生产中的应用奠定了基础。

Rice is one of the most important cereal crops.The introduction of dwarf rice has led to the first ＂green revo-lution＂.However,the genetic uniformity of dwarf gene in rice breeding is a concern,which severely affects the continuous increase in rice harvest.In the present study,we mutated the indica cultivar M804 by using 60Coγ-Ray and obtained a genetically stable rice dwarf mutant（MU101）,which was crossed with a japonica cultivar Taigeng 16 to investigate the genetic mode and map the gene.Genetic analysis revealed the phenotype of the dwarf mutant,which we designated tentatively as ds1,controlled by a pair of recessive genes.Polymorphic analysis of simple sequence repeat markers demonstrated the DS1 gene located on chromosome 5.With a larger mapping population and more Indel markers,we further mapped the DS1 gene between 2 Indel markers with a physical region of about 384 kb.These results provide a basis for molecular cloning and production application of DS1.