紫芫花化学成分研究

Study on the Chemical Constituents of Daphne genkwa

目的研究瑞香科植物紫芫花(Daphne genkwa Sieb.et Zucc.)干燥花蕾的化学成分。方法对紫芫花的体积分数95%乙醇提取物的乙酸乙酯部位采用硅胶、凝胶Sephadex LH-20等色谱手段进行化学成分的分离纯化,通过理化性质和波谱数据进行结构鉴定。结果分离得到12个化合物,鉴定了其中10个化合物,分别为芹菜素(apigenin,1)、芫花素(genkwanin,2)、刺槐素(4'-O-methylapigenin,3)、椴苷[kaempferol,3-O-β-D-(6-O-trans-p-coumaroyl)-glucopyranoside,4]、对羟基苯甲醛(4-hydroxybenzaldehyde,5)、对羟基桂皮酸甲酯(trans-p-hydroxy cinnamic methyl ester,6)、对羟基苯甲酸(p-hydroxybenzoic acid,7)、对羟基桂皮酸(p-hydroxy cinnamic acid,8)、苄基葡萄糖苷(benzyl-glucopyranoside,9)和苯乙基葡萄糖苷(phenylethyl-gluco-pyranoside,10)。结论化合物5～10为首次从该植物中分离得到。

To study the chemical consitituents of Daphne genkwa Sieb. et Zucc. METHODS The compounds were isolated by solvent extraction, and separated by silica gel and Sephadex LH -20 eolumn chromatography. Their structures were i- dentified by physicochemical properties and various spectroscopic methods including NMR spectrum, MS, UV,etc. RESUTLS Twelve compounds were isolated from the ethyl acetate part and 10 compounds were elucidated as apigenin（ 1 ）, genkwanin（2 ）,4＇-O-methyl- apigenin （ 3 ）, kaempferol [3-O-fl-D-（ 6-O-trans-p-coumaroyl ） -glucopyranoside ] （ 4 ）, 4-hydroxybenzaldehyde （ 5 ）, trans-p-hydroxy （ein- namic methyl ester （6） ,p-hydroxybenzoic acid （ 7 ） , p-hydroxy einnamic acid （ 8 ）, benzyl-glucopyranoside （9） and phenylethyl-glueopy- ranoside（ 10）, respectively. CONCLUSION Compounds 5 - 10 were isolated from Daphne genkwa for the first time.