摘要
目的:为准确鉴别南五味子和北五味子寻找一种新的鉴别手段。方法:采用RAPD-SCAR技术对南、北五味子基因组DNA进行PCR扩增。结果:100条RAPD引物中筛选出2条条带清楚、重复性好的RAPD引物能够区分南、北五味子,并将其转化为SCAR标记。结论:RAPD-SCAR技术是在分子水平上鉴别南、北五味子的一种行之有效的方法。
Objective: To establish a new method for the identification of Schisandra sphenanthera and S.chinensis.Method: Random amplified polymorphic DNA-Sequence characterized applied region(RAPD-SCAR) method was applied to screen primers.Result: Screening from 100 primers,only 2 random primers,which can be used to identify S.sphenanthera and S.chinensis accurately with a good reproducibility.It worked to fit them into sequence characterized applied region.Conclusion: RAPD-SCAR can be used to identify S.sphenanthera and S.chinensis accurately.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2011年第22期3083-3085,共3页
China Journal of Chinese Materia Medica
基金
辽宁省自然基金博士启动基金项目(20081067)
辽宁省自然科学基金项目(20072124)
关键词
南、北五味子
RAPD标记
SCAR标记
中药鉴定
Schisandra sphenanthera and S.chinensis
RAPD marker
SCAR marker
identification of herbal medicine