南、北五味子RAPD-SCAR鉴别研究

Identification of Schisandra sphenanthera and S.chinensis by random amplified polymorphic DNA sequence characterized applied region

目的:为准确鉴别南五味子和北五味子寻找一种新的鉴别手段。方法:采用RAPD-SCAR技术对南、北五味子基因组DNA进行PCR扩增。结果:100条RAPD引物中筛选出2条条带清楚、重复性好的RAPD引物能够区分南、北五味子,并将其转化为SCAR标记。结论:RAPD-SCAR技术是在分子水平上鉴别南、北五味子的一种行之有效的方法。

Objective： To establish a new method for the identification of Schisandra sphenanthera and S.chinensis.Method： Random amplified polymorphic DNA-Sequence characterized applied region（RAPD-SCAR） method was applied to screen primers.Result： Screening from 100 primers,only 2 random primers,which can be used to identify S.sphenanthera and S.chinensis accurately with a good reproducibility.It worked to fit them into sequence characterized applied region.Conclusion： RAPD-SCAR can be used to identify S.sphenanthera and S.chinensis accurately.