阳春砂仁种子质量检验方法的研究

Testing methods for seed quality of Amomum villosum

目的:研究阳春砂仁种子质量检验方法,为阳春砂仁种子检验规程和质量标准的制定提供依据。方法:参考国际植物种子检验规程和农作物种子检验规程的方法对不同产地阳春砂仁种子质量进行测定。结果:阳春砂仁净度分析送检样本最少500 g,试验样本最少50 g;真实性检验采用种子外观形态比较;质量测定采用百粒法;含水量测定采用高温烘干法3h;发芽前处理采用湿沙贮藏20 d后,用100 mg.L-1 GA3浸泡种子30 h,发芽以滤纸作发芽床,在30/20℃变温条件下萌发,发芽首末次计数时间为15～50 d;生活力测定采用TTC染色法。结论:初步建立了阳春砂仁种子扦样、净度分析、真实性鉴定、质量、含水量、发芽及生活力7项指标的检验方法。

Objective： The aim of this study was to optimize the testing methods for seed quality,and to provide a basis for establishing seed testing criterion and quality standard of Amomum villosum.Method： Referring to the International Seed Testing Rules made by ISTA and Rules for agricultural seed testing,the seed quality of A.villosum from different collection areas was measured.Result： The samples weight of A.villosum for purity analysis were at least 500 g and for test were at least 50 g.Verification of genuineness was assayed by seed appearance comparing and weight of per hundred seeds was determined,the moisture content test was carried out by high temperature drying method（3 hours）.The seeds were stored in wet sand for 20 days and then dipping in the 100 mg·L-1 GA3 for 30 days before germination,seeds on filter papers germinated at 30/20 ℃.The first germination-counting time was the 15th day of the test and the final time was the 50th day.Seed viability was tested by TTC method.Conclusion： the seed testing methods for quality items of A.villosum,including sampling,purity analysis,verification of genuineness,weight,moisture content,percentage germination and seed viability of A.villosum had been initially established.