UPLC同时测定荭草花中7种指标成分的含量

Simultaneous determination of seven compounds in flowers of Polygonum Orientale by ultra performance liquid chromatography coupled with photo diode array detection

目的:本研究采用UPLC建立了同时测定贵州产荭草花药材中没食子酸、儿茶素、高朦胧木素、花旗松素、山柰素-3-O-β-D-葡萄糖苷、槲皮素-3-O-α-L-鼠李糖苷、山柰素-3-O-α-L-鼠李糖苷7种指标成分的含量测定方法。方法:采用ACQU-ITY UPLC BEH C18色谱柱(2.1 mm×150 mm,1.7μm),流动相为0.1%甲酸乙腈-0.1%甲酸水溶液,梯度洗脱,流速为0.3mL.min-1,梯度洗脱,检测波长280 nm,柱温45℃。结果:7个指标成分在选定的范围内线性关系良好(r≥0.999 9),平均加样回收率(n=6)在97.90%～101.4%,RSD 2.3%～3.8%。结论:试验结果显示贵州产荭草花药材所含化学成分基本相同,但被测成分的含量有一定差异,8月中下旬采收的药材中被测成分的含量相对较高,质量较好。

To establish a UPLC method for simultaneous determination of seven chemical markers（gallic acid,catechin,alphitonin,taxifolin,kaempferol-3-O-β-D-glucoside,quercetin-3-O-α-L-rhamnoside,and kaempferol-3-O-α-L-rhamnoside） of the flowers of Polygonum Oriental collected from Guizhou province.Separation was performed at 45 ℃ on a ACQUITY UPLC BEH C18 column（2.1 mm×150 mm,1.7 μm） with a gradient solvent system of 0.1% formic acid in acetonitrile-0.1% aqueous formic acid as the mobile phase.The flow rate was 0.3 mL·min^-1,the detection wavelength was 280 nm,and the sample injection volume was 1 μL.In the selected linear range,all calibration curves of the seven chemical markers showed good linearity.The recoveries（n=6） were in the range of 97.90%-101.4% and RSD were less than 3.8%.The results showed that the seven compounds contained in all the samples of the flowers of P.orientale can be chosen as their chemical markers and that this UPLC-PAD method is simple,reliable and suitable for the quality control of the flowers of P.orientale.However,the chemical markers may be present at different levels for samples collected in different areas and at different times.A collection time of mid-to-late August with relatively high content of the chemical markers was suggested for the flowers of P.orientale.