双龙方配伍配比对大鼠急性心肌缺血模型的影响

Effect of compatibilities and proportions of Shuanglong formula on acute myocardial infarction in rats

目的:验证中药双龙方配伍的合理性和科学性。方法:通过盐酸异丙肾上腺素造成大鼠的急性心肌缺血模型,从生理层面(心电图)、病理层面(心肌梗死面积)和细胞分子层面(细胞酶学指标CK,LDH,SOD,MDA含量变化),观察单方以及双龙方不同配比预防给药对心肌缺血的拮抗作用。结果:各给药组均能改善心电图缺血指标、降低血清中CK,LDH,SOD,MDA含量及减少心肌梗死面积,但双龙方配伍高剂量组比其他组具有更好的效果,其中以3∶7高剂量组效果最优。结论:药效学筛选结果表明,按照双龙方配比3∶7高剂量对防治大鼠急性心肌梗死效果最优。

Objective： To verify the rational of Chinese medicine Shuanglong formula.Method： Rat models of acute myocardial ischemia were induced by hydrochloride isoproterenol.ECG J point change,myocardial infarction area and cellular enzyme（CK,LDH,SOD and MDA） levels were observed and detected to show protective effect of treatment groups.Result： The single drug prescriptions and compatibilities both could improve ECG performance,decrease levels of CK,LDH,SOD and MDA in serum,and reduce a certain myocardial infarct size.Conclusion： The Shuanglong formula at the high dose of 3∶7 was proved to be more effective on myocardial ischemia.