冰片单用及合用西药地西泮后对大鼠脑纹状体4种氨基酸类神经递质含量的影响

Effect of single-used borneol and combining it with diazepam on content of neurotransmitter in corpus striatum of rats

目的:探索开窍药冰片单用及合用西药地西泮后对大鼠脑纹状体区兴奋性及抑制性神经递质含量的影响,以期从神经药理学角度探讨冰片对中枢神经系统的作用,观察冰片是否可以协助西药入脑,增加其疗效。方法:利用脑微透析技术采集给药后不同时间段大鼠脑纹状体区的脑脊液样品,运用邻苯二甲醛柱前衍生反相高效液相色谱法,磷酸盐梯度洗脱结合荧光检测器检测样品中兴奋性神经递质天门冬氨酸(Asp)、谷氨酸(Glu)及抑制性神经递质甘氨酸(Gly)、γ-氨基丁酸(GA-BA)的含量,并进行统计分析。结果:与单用地西泮组相比,合用冰片后大鼠脑纹状体区兴奋性神经递质Asp及Glu含量变化不明显,抑制性神经递质Gly及GABA明显升高。结论:冰片对地西泮透过血脑屏障有一定的促进作用。

Objective： To research the content changes of excitatory neurotransmitter and inhibitory neurotransmitter in corpus striatum of rats after single-used borneol and combining it with diazepam in hope of comprehending the activity of borneol on central nervous system and to observe whether borneol could increase the penetration of other drugs into the brain.Method： The content of four amino acids neurotransmitters in corpus striatum of rats were sampled by brain microdialysis technology at different time after administration and were determined by RP-HPLC which involved pre-column derivation with orthophthaladehyde（OPA）,using phosphate gradient elution and fluorescence detection to detect the content of excitatory neurotransmitter aspartate（Asp）,glutamate（Glu） and inhibitory neurotransmitter glycine（Gly）,γ-aminobutyric acid（GABA） in standards and samples and carry on statistical analysis.Result： The content of both Gly and GABA in corpus striatum of rats with borneol increased significantly,compared with diazepam group（P〈0.05）,while Asp and Glu showed no significant difference.Conclusion： Borneol can improve permeability of diazepam through BBB.