中药组分清除烟气有害物质及降低细胞毒性的研究

Research of herb components on scavenging harmful components and reducing cytotoxicity of cigarette smoke

目的:研究中药组分体外清除多环芳烃、亚硝胺和自由基等烟气有害物质以及降低烟气细胞毒性的作用,为中药组分用于减少烟气危害发挥保健作用提供研究依据。方法:采用分光光度法检测中药组分对DPPH自由基、超氧阴离子和羟自由基的清除作用;以荧光光谱法测定各组分对多环芳烃的清除作用;以紫外分光光度法测定各组分对挥发性亚硝胺形成的抑制作用;并与抗坏血酸的作用进行比较;采用MTT法检测中药组分影响烟气造成的BEP2D细胞毒性作用。结果:所有中药组分均表现出一定的清除上述多种有害物质的作用,其综合清除能力以银杏叶提取物、甘草总黄酮、原花青素、麦冬总皂苷、淫羊藿总黄酮和黄芪总苷为好。以上6种中药组分可显著降低烟气造成的BEP2D细胞的细胞毒性,表现为细胞活存率(SF)显著上升,细胞平均致死剂量(DML)显著增加。结论:上述中药组分有良好的清除多环芳烃、亚硝胺和自由基等烟气有害致癌物质的作用,在减少烟气对人群的危害和预防烟草引起的各种疾病的发生中可发挥重要保健作用。

Objective：To study the in vitro effect of herb components on scavenging harmful components of cigarette smoke such as radicals,polycyclic aromatic hydrocarbons,nitrosamines in vitro,and its reducing effect on cytotoxicity of cigarette smoke.Method： spectrophotometry was used to examine the scavenging effect of herb components on DPPH free radicals,superoxide anion radical,and hydroxyl radical,and the results were compared with the anti-oxidation of ascorbic acid.Fluorescence spectroscopy was used to examine the scavenging effect of herb components on polycyclic aromatic hydrocarbons.UV spectrophotometry was used to examine the scavenging effect of herb components on volatile nitrosamines.MTT assay was used to examine cytotoxicity of cigarette smoke.Result： All the herb components showed a certain scavenging effect on DPPH free radicals,superoxide anion radical,hydroxyl radical,polycyclic aromatic hydrocarbons and volatile nitrosamines,espacially the ginkgo biloba extract（GBE）,flavonoids of glycyrrhiza,procyanidine,total saponins in ophiopogonin,total saponins of astragalus and epimediun flavonoids.After these six herb components were added to cigarette,the cytotoxicity of cigarette smoke on BEP2D cells was remarkably reduced,by increasing cell survival fraction（SF,%） and mean lethal dose（DML）.Conclusion： The herb components can scavenge harmful components of cigarette smoke such as radicals,polycyclic aromatic hydrocarbons and nitrosamines,which reduce the damage of cigarette smoke on human being.