摘要
目的 观察钙离子螯合剂对白血病患者骨髓单个核细胞（BMMNC）生成硫化氢（H2S）的影响.方法 采用BAPTA-AM和EGTA螯合细胞内外的钙离子,用敏感硫电极检测细胞外硫离子,计算出H2S含量的变化;免疫组化方法和图像分析系统检测bcl-2,bax的表达变化.结果 BAPTA-AM和EGTA各实验组与对照组比较,H2S生成量均明显降低（P〈0.05）.免疫组化结果显示,bcl-2和bax均表达于细胞浆和胞膜上.图像分析光密度值（OD值）,BAPTA-AM和EGTA各实验组bcl-2蛋白表达与对照组比较,12h,24h和48h均高于对照组（P〈0.05）.bax蛋白的表达在BAPTA-AM和EGTA各实验组与对照组相比,12h,24h和48h均低于对照组（P〈0.05）.结论 细胞内外钙离子螯合剂均能减少白血病患者BMMNC生成H2S,H2S生成降低在较短时间内可能抑制细胞凋亡,促进细胞增殖.
Objective To study the effect of calcium chelator on bone marrow mononuclear cells＇（BMMNC） capacity of producing endogenous hydrogen sulfide（ H2S} in patients with leukemia. Methods Abolishing calcium ion by using BAPTA-AM and EGTA,intracellular and extracellular calcium chelator to inhibit the production of endogenous H,S. Sensitive sulfion electrode was used to detect the concentration of sulfion in BAPTA-AM group medium, hnmunuhistochemical method and image analysis system were used to detect the expression of hel-2 and bax. Results lntracellular and extracellular calcium chelator could inhibit the production of endogenous H2S. Compared with the control group,the production of endogenous H2S decreased in BAPTA-AM and EGTA experimental group（ P 〈 0.05}. Results of immunohistochemisty, indicated that the expression level of bcl-2 in BAPTA-AM and EGTA group was higher than that of the control group（ P 〈 0.05 ）. The expression level of bax in BAPTA-AM and EGTA group was lower than that of the control group（ P 〈 0.05 ）. Conclusion Intracellular and extracellular calcium chelator could inhibit the production of endogenous H2S of BMMNC in patients with leukemia. Endogenous H2S could induce apoptosis of BMMNC in patients with leuke,nia. Inhibiting the production of endogenous H2S by using BAPTA-AM and EGTA could induce the decrease of apoptosis and increase of cell proliferation in short time.
出处
《潍坊医学院学报》
2011年第3期161-164,共4页
Acta Academiae Medicinae Weifang
基金
潍坊医学院研究生创新基金和出国留学基金资助项目（项目编号：YC2008010）
关键词
钙离子螯合剂
白血病
细胞凋亡
H2S
Calcium chelator
Leukemia
Cell apoptosis
Hydrogen sulfide
