大肠癌中ASPP1和p53蛋白的表达及意义

Expression and Significance of ASPP1 and p53 Protein in Colorectal Carcinoma

目的 观察人大肠癌组织中ASPP1以及p53蛋白的表达,并探讨其与大肠癌临床病理之间的关系.方法 应用免疫组织化学PV-6000法检测30例大肠癌和15例相应癌旁组织中ASPP1及p53的表达情况,比较ASPP1在大肠癌组织与癌旁组织中表达的差异,并探讨其与p53表达及临床病理之间的关系.结果 p53阴性时,大肠癌旁组织中ASPP1蛋白表达阳性率为80%,明显高于癌组织的16.7%（P〈0.05）;p53阳性时,癌组织、癌旁组织中的ASPP1蛋白表达差异无统计学意义（P〉0.05）.结论 当p53阴性时,大肠癌组织中ASPP1表达明显降低,这提示ASPP1蛋白表达降低在大肠癌的发生、发展中起着重要作用.

Objective To observe the expression of ASPP1 and p53 protein in coloreetal careinoma,and further to explore their relationship with clinical pathology characteristics. Methods Immunohistoehemical PV-6000 method was used to detect the expression of ASPP1 and p53 in 30 patients with eolorectal carcinoma and 15 samples of adjacent cancer,and their relationship with p53 expression and clinical pathology characteristics of colorectal carcinoma were studied. Results The expression rate of ASPP1 in p53-negative adjacent colorectal carcinoma tissues was 80% ,which was greatly higher than that in the colorectal carcinoma（ 16.7% , P 〈 0.05 ）. ASPP1 protein expression in colorectal carcinoma was not significantly different from that in the adjacent cancer tissues when the expression of p53 was positive （ P 〉0.05）. Conclusion The expression level of ASPP1 in p53-negative colorectal carcinoma tissues is obviously low, which indicates that the declined expression level of ASPP1 may be an important cause in the genesis and development of colorectal carcinoma.