摘要
目的:以酯键链接增强基团,制备姜黄素前体,观察其对前列腺癌细胞和正常二倍体细胞生长活性影响的差异。方法:叔丁氧羰基(Boc)-苯丙氨酸酯姜黄素单酯(BPC)作用于人前列腺癌DU-145细胞6~24 h后,MTT试验检测细胞生长活性,流式细胞术检测细胞凋亡率。计数法测定1~7天细胞生长曲线。人主动脉平滑肌(people aortic smooth muscle cells,HASMC)细胞作为对照组。结果:10~40μmol/L的BPC作用于DU-145细胞6~24 h后,DU-145细胞生长抑制率为7.37%~66.87%(P<0.05),呈浓度﹑时间依赖性;部分细胞出现凋亡形态学改变,FSC-SSC散点图可见24 h DU-145细胞凋亡比率为37.84%~47.12%(P<0.05)。对照组HASMC细胞凋亡比率为0.94%~4.23%(P<0.05),较同浓度姜黄素降低。结论:BPC能在体外有效诱导人前列腺癌DU-145细胞凋亡,对正常二倍体细胞的抑制作用较低,为深入研究泌尿系肿瘤靶向性治疗提供了新的途径。
Objective: Produced curcumin's precursors with toughen genes with ester key,to observe its different effect on growing cells between prostates' and the normal diploid's.Methods: Human prostatic cancer DU-145 cells were cultured and incubated with Boc-phenylalanine-curcumin(BPC) for 6-24 h.Cell viability was detected by MTT.Cell apoptosis rate was detected by flow cytometry.DU-145 cells were cultured and incubated with BPC for 1-7 d for cell grow curve detected by counting process.The people aortic smooth muscle cells(HASMC) acted as control.Results: After treatment with 10~40 μmol/L BPC for 6-24 h,the growth inhibition of DU-145 cells was 7.37%~66.87%(P 0.05),in dose-time-dependent manners.Partial cancer cells presented morph-ological characters of apoptosis,with FSC-SSC scatter diagram.After treatment for 24 h,the cell apoptosis rate was 37.84%~47.12%(P 0.05).Compared with curcumin of same concentrations,the control group HASMC apoptosis rate was 0.94%~4.23%(P 0.05).Conclusion: The prodrug of curcumin could induce apoptosis of prostatic cancer DU-145 cells,and reduce the side effects on normal diploid cells.This provided a novel strategy for further exploration of tumor-targeted chemotherapeutic drugs.
出处
《南京医科大学学报(自然科学版)》
CAS
CSCD
北大核心
2011年第10期1403-1406,1416,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家自然科学基金(30860284)
贵阳市科学技术攻关项目(2009筑科农合同字第3-009号)资助