摘要
对液相色谱法测定山楂片中苯甲酸、山梨酸、糖精钠进行了方法优化,对色谱条件进行了调整,采用ODS—Cl8型(150 mm×4.6 mm,5μm)色谱柱,流动相甲醇—0.02 mol/L乙酸铵(体积比5∶95),流速为1.0 mL/min,采用紫外检测器,检测波长为230 nm。方法测定结果的相对标准偏差小于2%(n=4),平均回收率大于90%,该法满足实验要求。
The determination of benzoic acid（sodium） ,sorbic acid（ potassium）, saccharin sodium in haw slices by high performance liquid chromatography was optimized. The conditiong of sample preparation was adjusted accordingly. ODS - C18 （ 150 mm x 4.6 mm） was used as column, with mobile phase 0.02 mol/L NH4Ac/methanol（95/5 ） and velocity of flow 1.0 mL/min. Wavelength was 230 nm determined by UV detector. The average recovery rate was above 90% , and the RSD was less than 2% （ n = 4）, which is suitable for the need of experiment.
出处
《粮油食品科技》
2011年第6期35-36,共2页
Science and Technology of Cereals,Oils and Foods
