肺癌中HDPR1的表达下调与肺癌的恶性程度相关

Downregulation of HDPR1 is associated with malignant phenotype of human lung cancer

目的研究HDPR1(human homologue of Dapper)在人非小细胞肺癌的表达,分析其表达与p120ctn和β-catenin表达是否相关,及其与肺癌恶性程度的关系。方法应用免疫组织化学染色检测120例非小细胞肺癌石蜡标本,应用WesternBlot和RT-PCR检测了42例新鲜组织标本中HDPR1、β-catenin和pl20ctn表达的关系,分析它们的表达与临床病理因素之间的相关性。结果 HDPR1在正常肺组织中为高表达,而在非小细胞中有68.3%(82/120)为表达下调,其表达下调不仅与肺癌高TNM分期、低分化、淋巴结转移和不良预后显著相关,并且与肺癌组织中p120ctn的表达下调呈正相关,与β-catenin的表达上调呈负相关。42例新鲜肺癌组织中,HDPR1和p120ctn的蛋白和mRNA均较癌旁正常组织显著下调(<0.05,n=42),然而,β-catenin蛋白在肺癌组织中却普遍表达上调(<0.05,n=42)。结论肺癌中HDPR1的表达下调与p120ctn和β-catenin异常表达相关,与患者的临床病理因素和不良预后明显相关。

Objective To study the expression of HDPR1（human homologue of Dapper）in nonsmall cell lung cancer（NSCLC）and analyze if the expression is correlated with β-catenin and p120-catenin（p120ctn）expressions and malignant degree.Methods Immunohistochemistry S-P method,RT-PCR and western blot were used to detect the expressions of HDPR1,p120ctn and β-catenin in lung cancer tissues.Results Immunohistochemical analysis showed that HDPR1 expression level was significantly higher in normal lung tissue than in NSCLC tissues（82/120,68.3%）,and reduced HDPR1 expression in NSCLC tissues was related to the clinicopathological factors and poor prognosis of the patients with NSCLC.In addition,reduced HDPR1 expression was positively correlated with the decreased expression of p120ctn,and negatively correlated to increased expression of β-catenin in lung cancer tissues.Moreover,RT-PCR and western blotting analysis showed that the expression of HDPR1 mRNA and protein was lower in tumor tissues as compared to corresponding nontumorous tissues.Conclusion The downregulation of HDPR1 expression is associated with the abnormal expression of p120ctn and β-catenin,and correlated with the malignancy degrees of human lung cancer.