葛根素治疗大鼠脊髓缺血再灌注损伤的实验研究

The Effect and Mechanism of Puerarin Early Treatment of Spinal Cord Ischemia-reperfusion Injury

目的探讨葛根素治疗脊髓缺血再灌注损伤的最佳时间及机制。方法制作雄性SD大鼠脊髓缺血再灌注损伤模型,分别于损伤后1、2、4、6 h腹腔内注射葛根素(50 mg/kg)。再灌注开始后48 h测试大鼠的运动功能,并测定硫氧还蛋白(Trx)表达水平及细胞凋亡指数。结果损伤后1、2、4及6 h葛根素组治疗组大鼠的运动功能有改善。缺血再灌注损伤导致Trx表达水平下降,而葛根素可提高Trx-1和Trx-2 mRNA表达水平,并大幅降低细胞凋亡指数。结论脊髓缺血再灌注发生后6 h内给予葛根素可减轻缺血再灌注造成的损伤。葛根素的神经保护作用与Trx表达水平的提高及细胞凋亡的减少有关。

Objective To explore the optimal therapeutic timing and mechanism of puerarin treatment of spinal cord ischemia-reperfusion injury. Methods Tile spinal ischemia-mpeffusion injury was conducted in male Sprague-Dawley rats,and 50 mg/kg of puerarin was injeet- ed intraperitoneally at 1,2,4 and 6 h after the injury. Motor function was measured 48 h after mperfusion started. Thioredoxin （Trx） expression and apoptosis indices were determined. Results Improvement of motor function at 1,2, and 4 h was demonstrated in the animals with puerarin treatment. Isehemia-reperfusion injury resulted in a deerease in the expression of thioredoxin, while puerarin administration elevated the expression of Trx-1/Trx-2 mRNA. Apoptosis indices were signifeanfly reduced by puerarin administration. Conclusion We concluded that administration of puerarin within 4 h of spinal isehemia-repeffusion injury reduced ischemic repeffusion damage, and that the neuropmtective effect of puerarin involved an increase in the transcription of Trx and a reduction of apoptosis.