不动杆菌属菌株WX-19还原Cr(Ⅵ)与降解苯酚特性

A Study on Simultaneous Chromium(Ⅵ)-Reduction and Phenol-Degradation by Acinetobacter sp.WX-19

从制革废水中筛选到1株既能还原Cr(Ⅵ)又能降解苯酚的不动杆菌属(Acinetobacter)菌株WX-19。菌株降解苯酚和还原Cr(Ⅵ)的最适温度为30℃,最适pH为7.0～8.0。0.2～0.5 mol/L NO3-、SO42-和Cl-能够促进WX-19的生长及其对Cr(Ⅵ)的还原。利用PCR、克隆和基因测序的方法从WX-19中检测到苯酚羟化酶基因(GenBank No.JF730215),与Pseudomonas sp.DHS3Y的苯酚羟化酶基因的同源性为88.5%。在含5 mg/LCr(Ⅵ)的基础盐培养基中,菌株WX-19可以利用苯酚为唯一碳源生长,并还原Cr(Ⅵ);在含有葡萄糖的基础盐培养基中,添加苯酚有利于菌株WX-19的生长和Cr(Ⅵ)还原。

An Acinetobacter strain designated as WX-19,capable of utilizing phenol as a sole carbon source for reducing Cr（Ⅵ） in mineral salt medium,was isolated from the activated sludge of a tannery wastewater treatment reactor.The optimal temperature and pH for Cr（Ⅵ） reduction and phenol degradation were 30 ℃ and 7.0-8.0,respectively.In mineral salt medium,0.2-0.5 mol/L of NO3-,SO42-and Cl-could increase the Cr（Ⅵ） reduction of strain WX-19.The phenol hydroxylase gene（GenBank No.JF730215） was detected from strain WX-19,which was closely related to that of Pseudomonas sp.DHS3Y.Strain WX-19 could grow on phenol（100 mg/L） as sole carbon and energy source,and could reduce 2 mg/L of Cr（Ⅵ） simultaneously.In mineral salt medium containing glucose,addition of phenol was beneficial for both growth and Cr（Ⅵ） reduction of strain WX-19.