一种新的组织块培养新生大鼠雪旺细胞方法(英文)

Novel method of explant-culture of neonatal rat Schwann cells

目的:为了建立一种组织块培养雪旺细胞的有效方法,为周围神经组织工程修复的研究提供种子细胞。方法:本研究从新生大鼠的周围神经分离雪旺细胞,经组织块法培养并纯化,观察并记录其形态特征;经HE染色以及S-100免疫组化染色鉴定并计算其纯度。结果:结果显示,4 d后,获得的雪旺细胞突起呈双极或三极,S-100阳性细胞纯度达到95%以上。结论:上述结果表明该方法快速有效,所获得的雪旺细胞纯度高、活性好。

Objective： In order to set up an effective method of explant-culturing Schwann cells（SCs） and provide seed cells for the study of peripheral nerve tissue engineering.Methods： SCs were isolated from neonatal rats＇ peripheral nerve and purified based on different attachment rates.SCs＇ morphological features were observed and recorded.The HE staining and immunohistochemical staining of S-100 protein was performed to identify the SCs.Results： The results showed that 4 days later,more than 95% cultured cells,which appeared typical bi-or tripolar morphology,were S-100 positive.Conclusion： These results suggest that the method is fast and effective,and it yields a large quantity of high purified SCs.