冬凌草甲素对PC-3转移的抑制效应及其分子机制

Metastasis-inhibiting effect of Oridonin on PC-3 cells and its mechanism

目的探讨冬凌草甲素是否具有抑制PC-3细胞转移的能力及其分子机制。方法用不同浓度的冬凌草甲素作用于PC-3细胞，用体外驱化运动实验检测PC-3细胞运动驱化能力的变化；用实时荧光定量聚合酶链反应（PCR）方法检测Pc-3细胞中血管内皮生长因子（VEGF）、基质金属蛋白酶（MMP）-2和MMP-9mRNA表达的变化；用酶联免疫吸附试验（ELISA）检测Pc-3细胞上清VEGF、MMP-2和MMP-9浓度的变化。结果体外驱化运动实验证明冬凌草甲素能以浓度依赖性方式抑制Pc-3细胞转移，以40umol／L冬凌草甲素组差异有统计学意义（P〈0．01）；冬凌草甲素以浓度依赖性方式抑制PC-3细胞及其上清中VEGF、MMP-2和MMP-9的表达（P〈0．01）。结论冬凌草甲素具有抑制Pc-3细胞转移的能力，其机制可能与药物下调VEGF、MMP-2和MMP-9有关。

Objective To determine metastasis-inhibiting capabihty of Oridonin on PC-3 cells and to explore its mechanisms. Methods After PC-3 cells were incubated with different concentrations of Oridonin, the metastasis ability was analyzed through experiment of ehemotaxie migration. The mRNA expression levels of vascular endothelial growth factor （VEGF） , matrix metalloproteioase （MMP）-2 and MMP-9 were detected by using fluorescent quantitative polymerase chain reaction （PCR）. The expression levels of VEGF, MMP-2 and MMP-9 proteins were examined by using enzyme linked immunosorbent assay （ ELISA ）. Results Oridonin effectively inhibited the metastasis of PC-3 cells in a concentration- and timedependent manner, and there was significant difference between 40 umol/L group and other groups （ P 〈 0. 01 ）. Oridonin effectively inhibited the expression levels of VEGF and MMP-9 in a concentration- and time-dependent fashion （ P 〈 0. 01 ）. Conclusion Oridonin can inhibit metastasis of PC-3 cells effectively in a concentration-dependent manner probably by down-regulating the expression of VEGF, MMP-2 and MMP-9.