小鼠肾缺血性损伤微小RNA表达谱的变化

The expression profile of microRNAs in ischemic kidney in mice

目的建立缺血肾组织微小RNA（miRNA）差异表达谱。方法夹闭小鼠双侧肾蒂45min制备急性肾缺血模型，随机分为假手术、缺血恢复4、24h组。通过观察血清肌酐（Scr）、尿素氮（BUN）及肾病理改变判断模型成功。采用AgilentmiRNA基因芯片技术构建缺血肾组织miRNA表达谱，实时定量聚合酶链反应（qRT-PCR）验证miRNA-210、miRNA-92a表达变化。结果miRNA表达谱出现明显改变，76个miRNAs出现两倍以上的表达变化，其中40个miRNAs下调，36个miRNAs上调。肾缺血恢复4、24h后，miRNA-210表达分别上调（2．02±0．29）、（5．58±0．16）倍；miR-92a分别上调（3．23±0．74）、（1．53±0．33）倍（P〈0．05），与miRNA微阵列结果一致。结论肾缺血损伤后，miRNA表达谱发生改变，提示miRNA可能参与缺血性肾损伤病理生理过程的调控。

Objective To screen the miRNA differential expression profile between nomal and ischemic renal tissues in mice. Methods Male BALB/C mice were subjected to a standard renal ischemia to induce acute kidney injury after 45 min of bilateral renal artery clamping. Mice were allowed to recover for 4, 24 h after ischemia or sham-surgery. The serum creatinine （Scr） and blood urea nitrogen （BUN） levels were measured and histological changes of the kidney were observed as markers of kidney injury. The changes of microRNAs （miRNAs） expression in renal tissues were detected by using Agilent miRNA microarrays after 24 h of ischemia or sham operation. The expression of miRNA-210 and miRNA-92a was validated by quantitative real-time reverse transcription-polymerase chain reaction （qRT-PCR）. Results Seventy-six miRNAs exhibiting more than twofold differences were identified in the kidney isehemic injury. Among them, 40 miRNAs exhibited decreased expression levels and 36 miRNAs exhibited increased expression levels. The expression of miRNA-210 was increased significantly by 2. 02 ±0. 29 and 5. 58 ±0. 16 at 4, 24 h after reperfusion respectively （ P 〈 0. 05 ）, while miRNA-92a was increased significantly by 3.23 ± 0. 74 and 1.53 ±0. 33 respectively （ P 〈 0. 05 ）. Conclusion There exist the expression changes of miRNAs in response to kidney isehima in mice, suggesting the potential involvement of miRNAs in modulating the pathophysiologic process of renal ischemia injury.