微小RNA-199a在胃癌中的表达及临床意义

Clinical significance of microRNA-199a expression in gastric cancer

目的探讨miR-199a-3p和miR-199a-5p在胃癌中的表达及其与临床病理特征的关系。方法提取32例胃癌及其癌旁正常胃黏膜组织标本的总RNA，采用茎环逆转录实时定量聚合酶链反应（PCR）方法检测miR-199a-3p和miR-199a-5p的表达量，并分析其与分化程度、淋巴结转移等临床病理特征的关系。结果与癌旁正常胃黏膜组织比较，miR-199a-3p和miR-199a-5p在癌组织中高表达（表达值中位数分别为2．07和1．97，P〈0．05），其中miR-199a-3p的表达与胃癌的淋巴结转移状态（P〈0．01）、肿瘤分化程度（P〈0．01）及TNM分期（P〈0．05）相关；miR-199a-Sp的表达与肿瘤分化程度（P〈0．01）及肿块部位相关（P〈0．05），ROC曲线分析显示，miR-199a-3p和miR-199a-5p评价胃癌细胞分化程度的特异性和敏感性均为71．4％和93．3％。结论miR-199a-3p和miR-199a-Sp在胃癌中的异常表达可能与胃癌的发生发展有关。

Objective To＇investigate the expression of miR-199a-3p and miR-199a-5p and its as- sociation with clinicopathologic features in gastric cancer. Methods The expression levels of miR-199a-3p and miR-199a-Sp in 32 gastric cancers and their matched non-tumor adjacent tissue specimens were detected by using stem-loop real-time reverse transcription-polymerase chain reaction （ RT-PCR）, and their correlation with clinicopathologic features of gastric cancer was analyzed. Results Expression levels of miR- 199a-3p and miR-199a-Sp were significantly higher in tumor tissues than in adjacent normal tissues （P 〈 0.05 ）. The expression of miR-199a-3p was associated with lymph node metastasis positively （P 〈 0.01 ）, TNM stage （P 〈 0. 05 ） and cell differentiation （P 〈 0.01 ）. The expressions of miR-199a-5p was associated with cell differentiation （ P 〈 0. 01 ） and location （ P 〈 0. 05 ）. ROC curve of miR-199a-3p and miR- 199a-5p about cell differentiation resulted in a diagnostic sensitivity of 93.3% and spectificity of 71.4%. Conclusion The level of miR-199a-3p and miR-199a-5p expression may be a valuable adjuvant parameter in forecasting the poorly differentiated gastric cancer.