防已黄芪汤对肺缺血再灌注损伤的保护作用

Protective effects of Fangji-huangqi decoction on lung ischemia-reperfusion injury

目的观察防已黄芪汤对兔肺缺血再灌注损伤的保护作用。方法将36只日本大耳白兔随机分为3组，每组12只，A组为对照组，B组为黄芪汤组，C组为防已黄芪汤组。制备兔在体肺缺血再灌注损伤模型。恢复灌注后4h（W4）、6h（T6）检测各组血清c反应蛋白（CaP）、超氧化物歧化酶（SOD）、丙二醛（MDA）和白细胞介素-6（IL-6）水平。结果T4、T6时段，SOD（B组：119．52±11．23、123．40±12．15；C组：125．34±12．56、129．38±11．54）较A组增加；MDA（B组：2．91±0．46、2．53±0．35、C组：2．85±0．32、2．56±0．24）、CRP（B组：2．89±0．17、2．37±0．13；C组：2．57±0．12、2．10±0．11）、IL-6（B组：697．24±47．62、654．35±37．21；C组：662．63±45．67、612．54±39．72）较A组均下降，差异有统计学意义（P〈0．01）；C组较B组MDA、SOD差异无统计学意义（P〉0．05），CRP、IL-6明显改善（P〈0．05）。结论防已黄芪汤能明显减轻兔肺缺血再灌注损伤，其作用机制可能与抗氧化、清除氧自由基及降低全身炎症反应有关。

Objective To study the pretective effect of the Fangji-Huangqi decoction on lung ischemia-reperfusion injury （IRI）. Methods Thirty-six Japanese rabbits were randomly divided into three groups： IRI group （group A）, Huangqi decoction group （group B） and Fangji-Huangqi decoction group （ group C）. Rabbit lung IRI model was established in vivo. The C-reactive protein （ CRP）, superoxide dismutase （ SOD）, malondialdehyde （MDA） and interleukin （IL） -6 of serum were determined at 4th （ T4 ） and 6th h （T6） after reperfusion. Results At T4 and T6, as compared with group A, SOD levels （ B ： 119. 52 ± 11.23, 123.40± 12. 15 ; C ： 125.34 ± 12. 56, 129. 38 ± 11.54） were higher, while MDA levels （B： 2.91 ±0.46, 2. 53 ±0. 35; C： 2. 85 ±0. 32, 2. 56 ±0. 24）, CRP levels （B： 2. 89 ±0. 17, 2. 37 ± 0.13; C： 2.57 ±0.12, 2.10 ±0.11）, and IL-6 levels （B： 697.24 ±47.62, 654.35 ±37.21; C： 662. 63 ±45.67,612. 54 ±39. 72） were lower （P〈0. 01 ） in groups B and C. There was no statistically significant difference in MDA and SOD levels between group B and group C （ P 〉 0.05 ）. The CRP and IL-6 levels were significantly improved in group C as compared with group B （ P 〈 0. 05 ）. Conclusion Fangji-Huangqi decoction can obviously alleviate lung IRI in rabbits probably by antioxidation, removal of oxygen radicals and reduction of systemic inflammatory response.