真空等离子喷涂Ti基层的HA＋Ti阶梯式涂层的犬人工股骨柄与骨的结合性能

Experiment study on osteointegration of prosthesis with Ti-HA graded coating being produced on pure titanium coatings in vacuum by plasma spraying

目的探讨基于真空等离子喷涂人工股骨柄钛（Ti）基层后复合羟基磷灰石（HA）．钛阶梯涂层界面与骨的结合性能。方法6条健康成年Beagle犬双侧髋关节人工股骨头置换术，置人大气等离子喷涂n基层结合纯HA涂层（HA＋Ti＋A）组，真空等离子喷涂Ti基层的HA-Ti阶梯式涂层（Ti＋HAG＋Ti＋V）组和真空等离子喷涂Ti基层结合纯HA（HA＋Ti＋V）组人工股骨柄各4个，术后28周行X线摄片、拔出实验、组织学观察及骨界面形态计量学分析。结果术后第3天全部犬恢复站立、行走。观察期间人工关节无松动及脱位。（Ti＋HAG＋Ti＋V）组剪切强度为（23．43±0．37）MPa，（HA＋Ti＋V）组的剪切强度为（20．07±1．95）MPa，（HA＋Ti＋A）组为（16．04±0．95）MPa。比较3组的压强值，差异有统计学意义（P〈0．05）。术后28周，（Ti＋HAG＋Ti＋V）组骨界面主要以部分矿化的类骨质及钙化良好的成熟骨组织充填，（Ti＋HAG＋Ti＋V）组每单位高倍视野下成骨细胞和骨细胞数高于（HA＋Ti＋V）组（t=9．98，P〈0．05）和（HA＋Ti＋A）组（t=15．16，P〈0．05），差异有统计学意义。结论基于真空下犬人工股骨柄Ti基层复合HA＋Ti阶梯式涂层在负重条件下骨一假体界面骨结合度好，强度高，具备应用于新型涂层假体研制的生物学性能。

Objective To evaluate bone ingrowth characteristics of a new prosthesis with Ti-HA graded coatings being produced on pure titanium coatings by plasma spraying. Methods The 12 hips from 6 adult health beagle dogs were divided into three groups （n = 4 each）, and transplanted with the following prostheses ： hydroxyapatite coatings on pure titanium coatings under atmosphere （ HA ＋ Ti ＋ A group）, hydroxyapatite coatings on pure titanium coatings under vaccum （ HA ＋ Ti ＋ V group）, and HA-Ti graded coatings under vaccum （Ti ＋ HAG ＋ Ti ＋ V group）. The animals were sacrificed at 28th week after operation, and the histological changes and the interface shear strength were evaluated. Results Position and function of the artificial prostheses in all groups were all satisfactory after replacement. No loose prostheses were found. Histologically, there was a good interface osteointegration in the three groups. Shear strength was （16.04±0.95） MPa in HA＋Ti ＋A group, （20.07 ±1.95） MPa in HA ＋Ti ＋V group, and （23.43 ± 0. 37 ） MPa in Ti ＋ HAG ＋ Ti ＋ V group respectively with the difference being statistically difference among three groups （ P 〈 0.05 ）. On the 28 week postoperatively, the interface in Ti ＋ HAG ＋ Ti ＋ V group was filled with calcified mature bone and partly mineralized osteoid tissue. The number of osteoblasts and osteocytes under a high powered field was significantly greater in Ti ＋ HAG ＋ Ti ＋ V group than in HA ＋ Ti ＋ V group （t=9.98, P〈0. 05） and HA＋Ti＋A group （t=15.16, P〈0.05）. Conclusion Ti-HA graded coatings on pure Ti coatings under vaccum showed good osteointegration and bonding strength, which obviously improved the stability of the prosthesis in weight loading.