大鼠体外膜肺氧合模型的建立

Establishment of rat model of extraeorporeal membrane oxygenation

目的建立大鼠体外膜肺氧合（ECMO）模型。方法选用成年健康雄性SPF级SD大鼠，利用特制微型动物膜式氧合器，经右颈静脉插管引流，尾动脉插管灌注建立小预充量的ECMO，转流时间120min，股动脉插管监测血流动力学指标及血气、电解质的变化。结果90％大鼠顺利建立ECMO模型，与ECMO前比较，平均动脉压（MAP）、红细胞压积（Hct）在ECMO30min、ECMO60min、ECM0120min下降（P〈0．05或P〈0．01），动脉血气及电解质指标差异无统计学意义（P〉0．05），基本达到理想的ECMO标准。停机后呼吸和心血管循环功能顺利恢复，获得了24h的较长时间生存。结论本方法建立的大鼠ECMO模型切实可行，简单实用、重复性好。

Objective To establish a clinically relevant rat extracorporeal membrane oxygenation （ECMO） model that would be a valuable tool for investigating pathophysiological and therapeutic strategies on bypass. Methods 20 male SD aged rats were anesthetized and intubated. The right external jugular vein,femoral artery and tail artery were cannulated. The blood was drained from the right atrium via the right jugular vein and further transferred by a small roller pump to a miniaturized hollow fiber oxygenator and return to the systemic circulation through the tail artery. ECMO were performed for 120 min at a flow rate of 80 to 120 ml/（ kg, rain）. During the whole process, the hemodynamics, electrocardiograph （ECG） and blood-gas analysis were observed. Results 18 of the 20 animals were performed successfully. Com- pared with before ECMO, MAP and Hct were decreased after ECMO（P 〈 0. 05 or P 〈 0. 01 ）. The blood-gas analysis at different time points were within a normal range. After ECMO weaning, cardiovascular and respiratory functions recovered gradually. Conclusion We have successfully established a miniature circuit consisting of prime for a rat ECMO model that maintains clinically acceptable results regarding hemodynamic parameters, blood gases, and hemodilution. This model would be valuable for further use in clinically relevant research studies.