组织工程PHBHH多孔材料喉支架的制备与细胞相容性研究

Fabrication of Larynx-Shape Porous PHBHH for Cartilage Tissue Engineering and the Cytocompatibility with Chondrocytes

目的探索组织工程多孔生物材料喉支架形态塑形物的制备技术,体外观察其细胞相容性,为进一步探寻软骨组织工程适宜生物材料提供实验依据。方法以新型生物材料聚羟基丁酸酯与聚羟基己酸酯共聚物[poly(3-hydroxybutyrate-co-3-hydroxyhexanoate),PHBHH,M=60万]为原料,氯化钠盐粒为致孔剂,采用溶剂浇铸、模压成形和颗粒滤沥技术制备多孔PHBHH喉支架形态塑形物,以乙醇置换法测定其孔隙率,负载软骨细胞后体外共同培养,通过大体形态、扫描电镜观察材料模压成形效果、材料孔隙连续性及其与软骨细胞粘附、分布和生长情况。结果模压成形的组织工程PHBHH材料塑形物类似喉支架形态,滤沥除盐后整体结构呈多孔海绵状,具有良好的支撑性,孔隙率达92%±2%,孔隙间连续性好,软骨细胞与材料粘附、生长及孔隙内分布状况良好,基质分泌旺盛。结论以溶剂浇铸、模压成形和颗粒滤沥技术可制备出支撑度和孔隙率适宜的PHBHH组织工程多孔材料的喉支架,其材料与软骨细胞体外培养相容性良好。

Objective To explore the possibility of using [poly（3-hydroxybutyrate-co-3-hydroxyhexanoate）,PHBHH] as biomaterials for cartilage tissue engineering of larynx and observe the cytocompatibility between PHBHH and chondrocytes.Methods Larynx-shape porous PHBHH models was fabricated by means of solvent casting,compression molding and particulate leaching techniques;and the porosity of PHBHH was measured by liquid（alcohol） replacement.The larynx-shape PHBHH models were evaluated by morphological observation.PHBHH porousness,and the attachment,growth condition and distribution between PHBHH and chondrocytes were evaluated by morphological observation and scanning electron microscopy.Results Larynx-shape porous PHBHH models with edges and corners of laryngeal cartilage appeared to be hollow half-trumpet shape and its porosity was 92%±2%.The porous PHBHH looked like netwok.The chondrocytes equallyl attached to the surface of porous PHBHH,filled within porousness,and secrected a large quantity of extracellular matrix.Conclusion Porous PHBHH may be used as biomaterials to fabricate larynx-shape tissue engineering models with suitable mechanical support and porosity,and had good cytocompatibility with chondrocytes in vitro.