肝癌组织中MIF、TNF-α的表达及临床意义

The expression and the clinical significance of MIF and TNF-α protein in human hepatocellular carcinoma

目的探讨巨噬细胞移动抑制因子(macro-phage migrationinhibitory factor,MIF)、肿瘤坏死因子(tumor necrosis factor-α,TNF-α)与肝癌的发生、发展及临床特征之间的关系。方法采用免疫组化法检测60例肝癌组织及20例癌旁正常对照组织中MIF和TNF-α的表达水平;分析上述指标与肝癌大小、有无远处转移、组织分化程度及其他临床资料之间的关系。结果 60例肝癌组织中MIF阳性者46例,阳性率为76.6%(46/60);TNF-α阳性者12例,阳性率为20%(12/60)。在20例癌旁正常组织中,MIF和TNF-α阳性例数分别为6例(30.0%)1、6例(80.0%)。MIF的表达与肝癌的分化程度、肿瘤大小、有无远处转移及癌栓形成呈正相关(P<0.05),TNF-α的表达与之相反(P>0.05)。结论 MIF和TNF-α与肝癌的发生、发展密切相关,MIF促进肝癌的发生、发展,TNF-α抑制肝癌的发生、发展。

Purpose To study the expression of macrophage migration inhibitory factors（MIF） and tumor necrosis factor-α（TNF-α） protein in human hepatocellular carcinoma（HCC） and the relationship with its clinical features.Methods The expression of MIF,TNF-α in 60 cases with HCC,20 cases with adjacent normal tissues as control group were detected by immunohistochemical technique.All patients were not treatment by chemotherapy,radiotherapy or other anti-cancer therapy before surgical operation.Results The positive expression rate of MIF protein in 60 cases of liver cancer tissues was 76.6%（46/60）,TNF-α protein positive expression rate was 20%（12/60）.In 20 cases of paracancerous benign liver tissue adjacent to cancer,MIF and TNF-α protein expression positive were observed in 6 patients（30.0%） and 17 cases（85.0%） respectively.MIF expression in carcinomas was higher than that in benign liver tissues,but TNF-α protein expression in benign liver tissues was significantly higher than cancerous tissue（P0.05）.The expression of MIF in cancers was positively related to the degree of differentiation,tumour size,distant metastasis and tumour embolus（P0.05）.In the contrast,the expression of MIF in cancers was negatively related to the degree of differentiation,tumour size,distant metastasis and tumour embolus（P0.05）.Conclusions MIF and TNF-α expression are closely related to the carcinogenesis and development of HCC.MIF may promote the carcinogenesis and development of HCC,TNF-α may inhibit the carcinogenesis and development of HCC.