大蒜素对骨肉瘤MG-63细胞系增殖和凋亡的影响

Effects of Garlicin on Proliferation and Apoptosis of MG-63 Cell Line

目的观察大蒜素对人成骨肉瘤细胞株MG-63增殖和凋亡的影响。方法不同浓度的大蒜素作用于体外培养的MG-63细胞,倒置显微镜下观察MG-63细胞的形态学变化,采用CCK-8法检测大蒜素对MG-63细胞增殖抑制能力,吖啶橙/溴化乙锭双荧光染色及Annexin V-FITC标记流式细胞术检测细胞凋亡情况以及对MG-63细胞周期影响。结果大蒜素可抑制人成骨肉瘤细胞株MG-63细胞增殖,具有明显剂量和时间依赖性,10μg/ml大蒜素可明显诱导MG-63细胞凋亡,并将细胞周期阻滞在G2/M期。结论大蒜素能够诱导MG-63细胞凋亡,阻滞细胞周期,抑制细胞增殖。

Objective To investigate the effects of garlicin on proliferation and apopotosis of human osteosarcoma cell line MG-63.Methods Under different garlicin concentrations,the MG-63 cells were observed by inverted microscope;cell counting of kit-8（CCK-8） assay was used to detect the growth inhibitory rates;and apoptosis was determined by acridine orange/ethidium bromide（AO/EB） double staining and Annexin V-FITC staining flow cytometry（FCM）.Cell Cycle was analyzed by FCM.Results Garlicin could significantly inhibit the growth of MG-63 in a dose and time dependent manner and induce G2/M phase cell cycle arrest.10 μg/ml garlicin could induce apoptosis obviously and arrest cell cycle at G2/M phase.Conclusion Garlicin was able to inhibit proliferation and induce apoptosis in MG-63 cells.