生姜提取物6-姜酚对对乙酰氨基酚致小鼠肝脏毒性的保护作用(英文)

6-Gingerol,an active ingredient of ginger,protects acetaminophen-induced hepatotoxicity in mice

目的:研究生姜提取物6-姜酚对对乙酰氨基酚致小鼠肝脏毒性的保护作用。方法:实验组小鼠在腹腔注射对乙酰氨基酚(900 mg/kg)30 min后,分别给予6-姜酚(30 mg/kg)或标准对照药水飞蓟素(25 mg/kg)。注射对乙酰氨基酚4 h后处死小鼠,检测血清中天冬氨酸氨基转移酶、丙氨酸氨基转移酶、碱性磷酸酶活性,总胆红素的含量及肝匀浆中脂质过氧化及抗氧化情况,如超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶、谷胱甘肽转移酶活性及还原型谷胱甘肽含量。结果:与对照组相比,6-姜酚及水飞蓟素均能显著降低对乙酰氨基酚引起的小鼠血清天冬氨酸氨基转移酶、丙氨酸氨基转移酶、碱性磷酸酶活性及总胆红素含量的升高(P<0.05)。此外,6-姜酚及水飞蓟素有效控制了肝脏内丙二醛的形成及各种抗氧化酶的降低(P<0.05)。结论:本研究的结果证实了6-姜酚具有与水飞蓟素相当的保肝作用。

Objective： To investigate the hepatoprotective efficacy of 6-gingerol against acetaminophen- induced hepatotoxicity in mice. Metheds： Mice were injected with a single close of acetaminophen （900 mg/kg） to induce hepatotoxicity, while 6-gingerol （30 mg/kg） or the standard drug silymarin （25 mg/kg） was given 30 rain after the acetaminophen administration. The mice were sacrificed 4 h after acetaminophen injection to determine the activities of liver marker enzymes such as aspartate aminotransferase （AST）, alanine aminotransferase （ALT） and alkaline phosphatase （ALP）, total bilirubin in serum, and lipid peroxidation and antioxidant status （superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione transferase and glutathione） in liver homogenate. Results： The treatment of 6-gingerol and silymarin to acetaminophen-induced hepatotoxicity showed significant hepatoprotective effect by lowering the hepatic marker enzymes （AST, ALT, and ALP） and total bilirubin in serum （P〈0.05）. In addition, 6-gingerol and silymarin treatment prevented the elevation of hepatic malondialdehyde formation and the depletion of antioxidant status in the liver of acetaminophen-intoxicated mice （P〈0.05）. Conclusion： The results evidently demonstrate that 6-gingerol has promising hepatoprotective effect which is comparable to the standard drug silymarin.