早期鸡胚发育过程中增殖细胞核抗原在法氏囊的表达

PCNA Expression in the Bursa of Fabricius(BF) in Chicken Early Embryo Development

法氏囊是鸟类特有的器官,是B淋巴细胞成熟的场所。本研究分析了鸡胚早期发育过程的中法氏囊形态变化及增殖细胞核抗原PCNA表达变化。结果表明鸡胚6.5d(E6.5)时,法氏囊开始出现在鸡胚泄殖腔的最深处,从最开始E6.5的一些小的囊泡发育成E8的一个大腔,E8～E10,法氏囊继续发育。从E11开始,法氏囊开始出现一些小的皱褶,E11～E18,这些小的皱褶逐渐变得清晰,并贯穿整个法氏囊。在E9时,法氏囊轮廓形成,可以发现在表皮层和基膜层PCNA有着广泛的表达。统计分析表明表皮层的PCNA阳性率在100%左右,在整个发育早期几乎没有变化;而基层的阳性率随着胚胎的发育逐渐下降,在E9、E10、E11、E12、E14和E18表达率分别是97%、93%、71%、55%、56%和20%。

The bursa of Fabricius（BF） is a special organ in birds,being the site of mature B lymphocytes.This study investigated proliferating cell nuclear antigen（PCNA） expression in the BF in chicken early embryo development（5 to 18 days）.The results showed that the BF of chicken embryos comes into being at the deepest point of the chicken cloaca at embryos of 6.5 days（E6.5）,commencing from small vacuoles that develop into one large capsule at 8 days（E8）,which continues growing from 8 to 10 days.The BF begins to exhibit small ruga in the capsule at E11,the ruga then become increasingly evident from 11 to 18 days,come through the whole BF,and the BF forms.When the BF profile formed at E9,with the strongest PCNA expression in the mucous epithelium,there is also broad PCNA expression in the lamina propria as the early embryo develops（9 to 18 days）,the relative percentages of PCNA-immunopositive cell accounting for the total mucous epithelium were 100%,100%,100%,99%,99% and 99% at E9,E10,E11,E12,E14 and E18,respectively;the relative percentages of PCNA-immunopositive cell accounting for the total lamina propria cells were 97%,93%,71%,55%,56% and 20% at E9,E10,E11,E12,E14 and E18,respectively,which suggesting that lamina propria cells proliferation of BF decrease gradually in chicken early embryo development.