蒙古马突触融合蛋白17(STX17)基因多态性及在不同毛色皮肤组织中的表达分析

Analysis on Polymorphism and Expression in Different Coat Color Skin of Syntaxin-17(STX17) Gene in Mongolian Horse

为研究突触融合蛋白17(STX17)基因与蒙古马毛色之间的相关性及其作用机制。本研究利用半巢式PCR技术及实时荧光定量PCR技术对蒙古马STX17基因第6内含子的多态性以及不同毛色蒙古马皮肤组织中STX17基因的相对表达量进行了检测。巢式PCR结果显示:STX17基因在青毛蒙古马第6内含子处存在4.6kb的重复片段,在其它毛色中不存在该重复片段;荧光定量PCR结果显示:STX17基因在蒙古马青毛皮肤组织中表达量最高,在骝毛皮肤组织中的表达量最低。结果证明蒙古马STX17基因与其青毛表型具有典型的相关性。

In order to research the relationship between syntaxin-17（STX17） gene and Mongolian horse coat color and mechanism,the polymorphism in intron 6 and the relative expression quantity in different colors skin of Mongolian horse were analyzed by semi-nested PCR and real-time quantitative PCR.The results showed that 4.6 kb duplication segments existed in intron 6 of STX17 gene in gray Mongolian horse,but not existed in non-gray Mongolian horse by semi-nested PCR.The expression level of STX17 gene was highest in gray skin and lowest in bay skin by real-time quantitative PCR.The results indicate that the STX17 gene has typical relationship with gray Mongolian horse.