胃癌相关激肽释放酶的筛选及其临床意义

Screening differentially expressed kallikrens in gastric cancer and its clinical significance

目的:筛选并探讨胃癌相关激肽释放酶(kallikrein,KLK)在胃癌中的表达,及其与胃癌临床病理和预后的关系。方法:用cDNA微阵列检测胃癌组织及正常胃黏膜标本,筛选出在胃癌组织中差异表达的KLK;用real-timePCR方法进一步验证;再用免疫组化检测胃癌组织石蜡切片中之目标KLK表达水平,并分析其与胃癌病人临床特征及预后的关系。结果:胃癌组织中,KLK10表达上调9.55倍,KLK11表达下调12.02倍。KLK10的表达水平与肿瘤直径、肿瘤浸润深度及淋巴结转移情况明显相关,而KLK11的表达水平与这些临床病理学特征均无关;肿瘤浸润深度、淋巴结转移情况及KLK10和KLK11表达状况与病人的总体生存率明显相关。结论:胃癌组织中差异表达的KLK主要包括KLK10及KLK11;KLK10的表达水平与肿瘤直径、肿瘤浸润深度、淋巴结转移情况及病人的总体生存率明显相关;而KLK11的表达水平与这些临床病理学特征均无相关,而与病人的总体生存率明显相关。

Objective To screen differentially expressed kallikrens in gastric cancer（GC） and assess the relationship between the expressing profiles of these genes with the patients′ clinical features and prognosis.Methods The differentially expressed kallikrens genes screened by cDNA microarrays were confirmed by real-time PCR in 10 pairs of cancerous and 10 pairs of noncancerous gastric tissues.The expressing profiles of these confirmed genes in GC were assayed by immunohistochemistry method,and the relationship between the expressing profiles with the patients′ clinical features and prognosis were assessed.Results KLK10 was up-regulated by 9.55 folds in GC,and KLK11 was down-regulated by 12.02 folds.The expressing profile of the KLK10 in GC was significantly associated with the tumor size,depth of tumor invasion and lymph node metastasis,while that of the KLK11 was not.Depth of tumor invasion,lymph node metastasis,and the expressing profiles of the KLK10 and KLK11 were all significantly associated with the patients′ overall survival.Conclusions KLK10 and KLK11 were differentially expressed in GC.The expressing profile of the KLK10 is significantly associated with the tumor size,depth of tumor invasion,lymph node metastasis and the patients′ overall survival,while that of the KLK11 is significantly associated with the patients′ overall survival only.