摘要
目的:探讨L-选择素、VLA-4α、LFA-1、ICAM-1、VCAM-1在IgA肾病(IgA nephropathy,IgAN)患者与非肾炎慢性扁桃体炎患者扁桃体的表达及意义。方法:收集10例伴有慢性扁桃体炎的IgAN患者扁桃体(IgAN组),这些患者经肾活检诊断为IgAN,10例不伴IgAN慢性扁桃体炎(Chronic tonsillitis,CT组)作为对照。应用Western blot技术定量分析两组扁桃体组织L-选择素,VLA-4α、LFA-1、ICAM-1、VCAM-1的表达,同时采用免疫组织化学技术检测扁桃体L-选择素、VLA-4α、LFA-1表达,观察ICAM-1,VCAM-1在生发中心和滤泡间区高内皮小静脉(High endothelial venules HEV)分布。结果:Western blot技术显示IgAN组及CT组扁桃体L-选择素、VLA-4α、LFA-1、ICAM-1、VCAM-1的表达分别为〔(1.10±0.09;0.80±0.14)、(0.39±0.05;0.24±0.04)、(0.41±0.06;0.29±0.06)、(1.36±0.04;1.18±0.09)、(2.56±0.63;1.60±0.32)]。免疫组织化学检测两组各归巢受体表达差别有显著统计学意义,IgAN扁桃体生发中心和滤泡外区HEV内血管地址素表达增强。结论:扁桃体淋巴细胞归巢异常是伴有慢性扁桃体炎的IgAN患者的重要特征,IgAN的发病机制与之有密切关联。
Objective:To investigate the expression of L-selectin,VLA-4α,LFA1,ICAM-1,VCAM-1 in the tonsil of patients with IgA nephropathy(IgAN).Methods:Studied the tonsil samples of 10 patients with biopsy-proven IgAN(IgAN group),(6 male,mean age 24±9) and 10 adults who had chronic tonsilitis without the history of kidney disease(CT group),(5 males,mean age 27±12).Western blot technique was used to analyze L-selectin,VLA-4α,LFA-1,ICAM-1,VCAM-1 expression in the tonsil.Also,using immunohistochemistry(SABC),we observed the expression of homing receptors L-selectin,VLA-4α,LFA-1 in the tonsils.In germinal centers and high endothelial venules(HEV),vascular addressin(ICAM-1,VCAM-1) was examined at the mean time.All data was fed into a personal computer using SPSS13.0 statistics software.Results:Western blot showed the statistically enhanced expression of L-selectin,VLA-4α,LFA-1,ICAM-1 and VCAM-1 in IgAN group compared with CT group(1.10±0.09 vs 0.80±0.14),(0.39±0.05 vs 0.24±0.04),(0.41±0.06 vs 0.29±0.06),(1.36±0.04 vs 1.18±0.09),(2.56±0.63 vs 1.60±0.32),respectively.On immunohistochemical analysis,changes in homing receptors of the tonsil were statistically significance,and there was the enhanced expression of the vascular addressins in the tonsillar germinal centers and HEV in IgAN group.Conclusion:While suffering from chronic tonsillitis,the patients with IgAN is characteristic of the increased expression of L-selectin,VLA-4α,LFA-1,ICAM-1,VCAM-1 in the tonsil.The pathogenesis of IgAN is closely related to lymphocyte abnormally homing to the tonsil.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2011年第9期832-836,839,共6页
Chinese Journal of Immunology
基金
湖北省卫生厅科研基金项目(JXIB043)