原花青素抗氧化活性及对细胞氧化损伤的保护作用

A study on antioxidant activity and protective effect to cellular oxidative damage of procyanidine

采用铁氰化钾法、DMPD.+法和SDS-PAGE法,检测了原花青素的还原力、对DMPD.+自由基的清除能力及蛋白质氧化降解的保护作用;采用SRB法和单细胞凝胶电泳法,检测了原花青素对细胞氧化损伤的保护作用.研究结果表明,原花青素在各体系中均具有较强的抗氧化作用,对蛋白质氧化降解、大鼠星形神经胶质细胞及人外周血淋巴细胞DNA损伤的保护作用逐渐增强,且呈浓度依赖关系.说明原花青素在不同的反应体系中总体表现出较强抗氧化活性,并对由H2O2诱导的细胞氧化损伤具有一定的保护作用.

To study the antioxidant activity of procyanidine,the K3Fe（CN）6,DMPD method and SDS-PAGE are used for the detection of the reducing activity,the scavenging action of DMPD·＋ and the protections against protein oxidation degradation.SRB assay and single cell gel electrophoresis are used to detect the protective effect to cellular oxidative damage.The results indicate that procyanidine exhibite evident antioxidant activities in different antioxidant systems.It shows obvious protections against the protein oxidation degradation,astrocyte cell and DNA damage of peripheral blood lymphocyte,and both in concentration-dependent.These results indicate that procyanidine exhibits significantly antioxidant activities in different reactive systems and an obvious protection effect on cellular oxidative damage by H2O2.