荣昌、长白、杜洛克猪肠道微生物ERIC-PCR-DGGE指纹图谱比较分析

Intestinal Microbial Community and Its Fingerprint Comparative Analysis between Rongchang,Landrace and Duroc using ERIC-PCR-DGGE

为了解荣昌猪与外种猪肠道微生物间的差异,通过提取荣昌、长白、杜洛克猪不同生长阶段粪样总DNA,进行ERIC-PCR扩增及其DGGE指纹图谱的比较分析。结果表明:ERIC-PCR-DGGE技术是一种有效的分子微生态分析技术,它能较好地反映猪肠道微生物群落构成。在哺乳、断奶和经产三个阶段的指纹图谱中,不同品种猪粪样微生物种/属相似性较高,但分布和数量存在较大的差异,表明猪肠道微生物群落多样性与品种不同密切相关。哺乳仔猪由于受母猪的影响,谱带差别明显;而断奶和经产阶段的图谱中,荣昌猪粪样包含的可见条带数量最多,提示其肠道中定植的微生物类群可能更高,由于肠道菌群在营养、免疫和生物拮抗等方面影响机体健康,以上结果为解释荣昌猪具有适应性好、抗逆性强、耐粗饲和抗病力强等特点提供一些佐证。

To analyze the flora and the difference of intestinal microbial community diversity between Rongchang and foreign breed swine,Enterobacterial Repetitive Intergenic Consensus（ERIC）-PCR-DGGE was applied to analyze the intestinal microbial community from healthy suckling piglets,weaning piglets and multiparous sows between Rongchang,Landrace and Duroc.The results showed that ERIC-PCR-DGGE is a useful method for investigation molecular micro-ecology and can monitor intestinal microbial community.In 3 DGGE fingerprints,high species/genus similarity exist in 3 breed swine＇s intestinal microbial communities,while there are obvious difference in the position and quantity,which shows that microbial flora closely related with swine breeds.Suckling piglet DNA bands show obvious diversity due to the sows influence,while the bands in the weaning and multiparous sows,Rongchang has the most bands among the 3 swine breeds,which meaning that there are the most intestinal microbial communities colonization in Rongchang intestinal tract compare with that of in the other two swine breeds＇.Because intestinal microbial community has obvious influence on host healthy in its nutrition,immunoresponsiveness,biological antagonistic.The study offers useful data for further interpretation of the good adaptivity,antireversion force,crude feed tolerance and strong disease resistance characteristics that Rongchang swine owned.