原子荧光法测定药用玻璃瓶中砷含量的研究

The Study of Arsenic Concentration in Medicine Glass by Using Atomic Fluorescent Spectrometry

探讨利用原子荧光法测定药用玻璃瓶中砷的含量,建立药用包装材料中砷含量的新型检测方法。药用玻璃瓶经5%盐酸溶液、98℃蒸煮2 h后,用硫脲-抗坏血酸作为混合还原剂将浸提液中的五价砷还原为三价砷,再与硼氢化钾反应生成砷化氢气体,载入原子荧光光度计,通过测定不同浓度的砷标准溶液,制作标准曲线,从而测定样品中的砷含量。本方法线性范围为0～20μg.L-1,相关系数r为0.992。两批药用玻璃瓶的砷含量均低于国家标准规定的限度(0.2 mg.L-1)。原子荧光法简便快速、灵敏度高、准确度好,优于传统的化学检测方法,适用于药品包装材料中砷含量的测定。

This study was designed to evaluate the method for concentration measurement of arsenic and set up a new assessment for arsenic in the packing material of medicine. Using glass bottle steamed boiled with 5% HC1 at 98 ℃for 2 h, 5-valence arsenic in the extraction solution was reduced to 3-valence arsenic by ThioureaAscorbic Acid and produced the gas of arsenic hydride after reaction with Potassium borohydrid. The gas of arsenic hydride was loaded into the fluorescence spectrophotometer. The standard solutions with dif~ ferent arsenic concentrations were made and standard curve was set up by atomic fluorescence spectrophotometer. Finally, the concentra- tion of arsenic in the samples was determined. The results showed that the linear range of this method was 0 ～ 20 p.g～ L L and the correlation coefficient was 0.992. The concentration of arsenic in both two groups glass bottles were lower than the limit of national standard （0.2 mg · L-1 ）. It suggests that the method of atomic fluorescence is rapid and high sensitive with good accuracy and it is better than the traditional chemical methods. Our method could be use to determine the arsenic concentration in drug pack materials.